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Rabbit Anti-phospho-DOK1 (Tyr362)  antibody (bs-5289R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-5289R
英文名称 phospho-DOK1 (Tyr362)
中文名称 磷酸化D酪氨酸激酶衰减蛋白1抗体
别    名 DOK1 (phospho Y362); DOK1 (phospho Tyr362); p-DOK1 (phospho Y362); docking protein 1 (downstream of tyrosine kinase 1); Docking protein 1 (p62(dok)) (Downstream of tyrosine kinase 1) (pp62).; docking protein 1 62kD (downstream of tyrosine kinase 1); Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK-1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62; pp62.  
产品类型 磷酸化抗体 
研究领域 肿瘤  心血管  信号转导  细胞凋亡  G蛋白偶联受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human (predicted: Mouse,Rat,Dog,Pig,Cow)
产品应用 WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 62kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr362: PI(p-Y)DE 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Function:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

Subunit:
Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.

Subcellular Location:
Isoform 1: Cytoplasm. Isoform 3: Cytoplasm, perinuclear region.

Tissue Specificity:
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.

Post-translational modifications:
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated by LYN.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
Isoform 3 contains a N-acetylmethionine at position 1.

Similarity:
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.

SWISS:
Q99704

Gene ID:
1796

Database links:

Entrez Gene: 1796 Human

Entrez Gene: 13448 Mouse

Entrez Gene: 312477 Rat

Omim: 602919 Human

SwissProt: Q99704 Human

SwissProt: P97465 Mouse

SwissProt: Q4QQV2 Rat

Unigene: 103854 Human

Unigene: 156 Mouse

Unigene: 117974 Rat



产品图片
Sample:
Jurkat(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-DOK1(Tyr362) (bs-5289R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
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