| 产品编号 | bs-4191R |
| 英文名称 | ADAMTS4 |
| 中文名称 | ADAMTS4抗体 |
| 别 名 | ATS4_HUMAN; ADAMTS 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4; A disintegrin like and metalloprotease with thrombospondin type 1 motif 4; ADAM metallopeptidase with thrombospondin type 1 motif 4; ADAM TS 4; ADAM TS4; ADAMTS-2; ADMP-1; ADAMTS4 protein; Aggrecanase1; KIAA0688. |
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Specific References (4) | bs-4191R has been referenced in 4 publications.
[IF=20.722] Fanqi Hu. et al. Identification of inflammatory regulation roles of thalidomide/ruxolitinib in nucleus pulposus and construction of polyelectrolyte nanocomplexes-impregnated injectable hydrogels for synergistic intervertebral disk degeneration treatment. Nano Today. 2022 Jun;44:101462 WB ; Human.
[IF=4.868] Liu Y et al. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxid Med Cell Longev. 2019 Nov 6;2019:7189854. ICF ; Human.
[IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human.
[IF=1.785] Tao Yang. et al. Vitamin D3 protects articular cartilage by inhibiting the Wnt/β‑catenin signaling pathway. Exp Ther Med. 2020 Aug;20(2):1775-1781 WB ; Rat.
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| 研究领域 | 肿瘤 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Sheep,Rabbit,Cow) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 90kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAMTS4: 501-839/839 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The expression of this gene is upregulated in arthritic disease and this may contribute to disease progression through the degradation of aggrecan. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu- -Ala-393' site. Subcellular Location: Secreted. extracellular space. extracellular matrix. Tissue Specificity: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Post-translational modifications: The precursor is cleaved by a furin endopeptidase. Similarity: Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 1 TSP type-1 domain. SWISS: O75173 Gene ID: 9507 Database links: Entrez Gene: 9507 Human Entrez Gene: 240913 Mouse Omim: 603876 Human SwissProt: O75173 Human SwissProt: Q8BNJ2 Mouse Unigene: 211604 Human Unigene: 475917 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Human A549 cell lysates Primary: Anti-ADAMTS4 (bs-4191R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 100 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
