| 产品编号 | bs-5918R |
| 英文名称 | ANGPTL3 |
| 中文名称 | 血管生成素样蛋白3抗体 |
| 别 名 | ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGL3_HUMAN; ANGPT5; FHBL2. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 生长因子和激素 血管内皮细胞 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ANG5/ANGPTL3: 21-120/460 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Angiopoietin-like protein 3 (Angptl3) functions as a potent lipoprotein lipase inhibitor and is an important component of plasma triglyceride homeostasis. Angptl3 also plays a role in adipose formation and angiogenesis through its interaction with integrin ?v)beta(3). It is secreted by the liver and is functionally defined by the C-terminal fibrinogen (FBN)-like domain and an N-terminal coiled-coil domain. Angptl3 regulates circulating triglyceride levels during different nutritional states thereby mediating the feeding/fasting cycle. A deficiency of Angptl3 results in abnormally low lipid levels, and a repression of the protein may be protective against atherosclerosis. Angptl3 may also play an important role in hyperlipidemia in diabetes. Subcellular Location: Secreted Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. Similarity: Contains 1 fibrinogen C-terminal domain. SWISS: Q9Y5C1 Gene ID: 27329 Database links: Entrez Gene: 27329 Human Entrez Gene: 30924 Mouse Omim: 604774 Human SwissProt: Q9Y5C1 Human SwissProt: Q9R182 Mouse Unigene: 209153 Human Unigene: 28341 Mouse |
| 产品图片 |
Sample:
Hepg2(Human) Cell Lysate at 30 ug Primary: Anti-ANGPTL3 (bs-5918R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 59 kD 
Sample:
Lane 1: Human HL-60 cell Lysates Lane 2: Human HepG2 cell Lysates Lane 3: Human 293T cell Lysates Lane 4: Human Raji cell Lysates Lane 5: Human MOLT4 cell Lysates Primary: Anti-ANGPTL3 (bs-5918R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49kDa Observed band size: 60kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
