| 产品编号 | bs-4256R |
| 英文名称 | Thioredoxin 2 Rabbit pAb |
| 中文名称 | 线粒体硫氧还蛋白2抗体 |
| 别 名 | mitochondrial thioredoxin; MT-TRX; MTRX; thioredoxin 2 precursor; Thioredoxin mitochondrial; Thioredoxin2; TRX 2; TRX2; TXN 2; TXN2; THIOM_HUMAN. |
| 研究领域 | 肿瘤 免疫学 肿瘤细胞生物标志物 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Thioredoxin 2: 101-166/166 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. Function: Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity. Subunit: Monomer. Subcellular Location: Mitochondrion. Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues. Similarity: Belongs to the thioredoxin family. Contains 1 thioredoxin domain. SWISS: Q99757 Gene ID: 25828 Database links: Entrez Gene: 25828 Human Entrez Gene: 56551 Mouse Omim: 609063 Human SwissProt: Q99757 Human SwissProt: P97493 Mouse Unigene: 211929 Human Unigene: 55043 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Small intestine tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human 293T cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human HeLa cell lysates
Primary: Anti-Thioredoxin 2 (bs-4256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin 2) Polyclonal Antibody, Unconjugated (bs-4256R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
