产品编号 | bs-4293R |
英文名称 | MACC1 Rabbit pAb |
中文名称 | 结肠癌转移相关蛋白1抗体 |
别 名 | 7A5; MACC1; MACC-1; MACC1_HUMAN; Metastasis associated in colon cancer 1; Metastasis-associated in colon cancer protein 1; Putative binding protein 7a5; SH3 domain-containing protein 7a5; SH3BP4L. |
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Specific References (6) | bs-4293R has been referenced in 6 publications.
[IF=5.008] Montorsi, Lucia, et al. "Loss of zfp36 expression in colorectal cancer correlates to wnt/ß-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of zeb1, sox9 and macc1." Oncotarget (2016). WB ; Human.
[IF=2.916] Shi Yan. et al. The Relationship between MACC1/c-Met/Cyclin D1 Axis Expression and Prognosis in ESCC. Anal Cell Pathol. 2022;2022:9651503 IHC ; Human.
[IF=2.74] Guo et al. Expression of MACC1 and c-Met in human gastric cancer and its clinical significance. (2013) Cancer.Cell.Int. 13:121 IHC ; Human.
[IF=1.69] Shi, Woda, et al. "MACC‑1 antibody target therapy suppresses growth and migration of non‑small cell lung cancer." Molecular Medicine Reports 16.5 (2017): 7329-7336. ELISA ; Human.
[IF=1.48] Sun, Longfeng, et al. "Silence of MACC1 expression by RNA interference inhibits proliferation, invasion and metastasis, and promotes apoptosis in U251 human malignant glioma cells." Molecular Medicine Reports. WB ; Human.
[IF=1.48] Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996. IHC-P ; Human.
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研究领域 | 肿瘤 免疫学 信号转导 生长因子和激素 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse,Rat (predicted: Cow) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 94 kDa |
检测分子量 | |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MACC1: 761-852/852 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
MACC1 is a key regulator of the hepatocyte growth factor(HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed19098908]).[supplied by OMIM]. Subunit: Interacts with FASLG. Subcellular Location: Cytoplasm. Nucleus. Note=Mainly found in the cytoplasm in non-metastasizing tumors. Tissue Specificity: Preferentially expressed in metastasizing tumors. Similarity: Contains 1 SH3 domain. SWISS: Q6ZN28 Gene ID: 346389 Database links: Entrez Gene: 346389 Human Entrez Gene: 238455 Mouse Omim: 612646 Human SwissProt: Q6ZN28 Human Unigene: 598388 Human Unigene: 31376 Mouse Unigene: 81938 Rat |
产品图片 |
Sample:
liver(mouse) Lysate at 40 ug
Primary: Anti-MACC1 (bs-4293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kD
Observed band size: 94 kD
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human A431 cell lysates
Lane 3: Human A549 cell lysates
Primary: Anti-MACC1 (bs-4293R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 97 kDa
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MACC1 Polyclonal Antibody, Unconjugated(bs-4293R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MACC1) Polyclonal Antibody, Unconjugated (bs-4293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |