| 产品编号 | bs-5762R |
| 英文名称 | S100-A8 / MRP8 |
| 中文名称 | 多药耐药相关蛋白8抗体 |
| 别 名 | 60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A; Calprotectin L1L subunit; Calprotectin, included; CFAG; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A8; S100 calcium-binding protein A8; S100A8; S100A8/S100A9 complex, included; S10A8_HUMAN; Urinary stone protein band A. |
| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human S100A8: 21-80/93 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]. Function: Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. Subunit: Homodimer. Heterotetramer with S100A9. Component of the heterotetrameric calprotectin complex containing two copies each of S100A8 and S100A9. Interacts with AGER and with the heterodimeric complex formed by TLR4 and LY96. Interacts with CEACAM3 and tubulin filaments in a calcium-dependent manner. Heterotetrameric calprotectin interacts with ANXA6 and associates with tubulin filaments in activated monocytes. May interact with components of the intermediate filaments in monocytes and epithelial cells. Subcellular Location: Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. Tissue Specificity: Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses. Similarity: Belongs to the S-100 family. Contains 2 EF-hand domains. SWISS: P05109 Gene ID: 6279 Database links: Entrez Gene: 6279 Human Entrez Gene: 20201 Mouse Omim: 123885 Human SwissProt: P05109 Human SwissProt: P27005 Mouse Unigene: 416073 Human Unigene: 21567 Mouse |
| 产品图片 |
Sample:
Lane 1: Recombinant human S100-A8/A9 protein, His(bs-41227P) Primary: Anti-S100-A8 / MRP8 (bs-5762R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 32 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
