| 产品编号 | bs-5913R |
| 英文名称 | Calreticulin |
| 中文名称 | 钙网蛋白抗体 |
| 别 名 | Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA. |
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Specific References (15) | bs-5913R has been referenced in 15 publications.
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| 研究领域 | 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calreticulin: 101-200/417 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] Subunit: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Subcellular Location: Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Similarity: Belongs to the calreticulin family. SWISS: P27797 Gene ID: 811 Database links: Entrez Gene: 41166 Fruit fly (Drosophila melanogaster) Entrez Gene: 811 Human Entrez Gene: 12317 Mouse Entrez Gene: 100009050 Rabbit Omim: 109091 Human SwissProt: P29413 Fruit fly (Drosophila melanogaster) SwissProt: P27797 Human SwissProt: P14211 Mouse SwissProt: P15253 Rabbit Unigene: 2457 Fruit fly (Drosophila melanogaster) Unigene: 515162 Human Unigene: 1971 Mouse Unigene: 467043 Mouse Unigene: 974 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Rat Testis tissue lysates Lane 4: Human SH-SY5Y cell lysates Lane 5: Human HeLa cell lysates Lane 6: Human K562 cell lysates Lane 7: Human HL-60 cell lysates Lane 8: Human A431 cell lysates Primary: Anti-Calreticulin (bs-5913R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 48 kDa 
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calreticulin Polyclonal Antibody, FITC conjugated (bs-5913R-FITC) 1:100, 40 minutes at 37°C. 
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Cell: Hela
Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-5913R) on Hela(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . 
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (bs-5913R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
