| 产品编号 | bs-5998R |
| 英文名称 | DAB2IP Rabbit pAb |
| 中文名称 | Dab2相互作用蛋白抗体 |
| 别 名 | AF9Q34; AIP1; ASK interacting protein; DAB2P_HUMAN; ASK interacting protein 1; ASK1 interacting protein 1; DAB2 interacting protein; DAB2 interaction protein; DAB2P; DIP1/2; Disabled homolog 2 interacting protein; DOC 2/DAB2 interactive protein; FLJ39072; KIAA1743; nGAP like protein. |
| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 132 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAB2IP: 901-1069/1069 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex. Function: Functions as a Ras GTPase-activating protein (By similarity). Through its interaction with MAP3K5 disrupts the association of MAP3K5 with the inhibitory 14-3-3 complex. Mediates TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK-NF-kappa-B signaling. May act as a tumor suppressor gene. Subunit: Interacts with MAP3K5, preferentially with its 'Ser-966' dephosphorylated form. On plasma membrane, exists in an inactive form complexed with TNFR1. In response to TNF, dissociates from TNFR1 complex, tranlocates to cytoplasm and forms part of an intracellular signaling complex comprising TRADD, RALBP1, TRAF2 and MAP3K5. Binding to MAP3K5 is induced by TNF. Interacts with DAB1 and DAB2 (By similarity). Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis. Subcellular Location: Membrane. Cytoplasm. Note=Translocates to the cytoplasm in response to TNF. Tissue Specificity: Low expression in prostate. Down-regulated in prostate cancer. DISEASE: Note=A chromosomal aberration involving DAB2IP is found in a patient with acute myeloid leukemia (AML). Translocation t(9;11)(q34;q23) with MLL. May give rise to a MLL-DAB2IP fusion protein lacking the PH domain. Similarity: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain. SWISS: Q5VWQ8 Gene ID: 153090 Database links: Entrez Gene: 153090 Human Entrez Gene: 69601 Mouse Omim: 609205 Human SwissProt: Q5VWQ8 Human SwissProt: Q3UHC7 Mouse Unigene: 522378 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
