产品中心-北京博奥森生物技术有限公司

产品编号	bs-6028R
英文名称	CD16
中文名称	FC段γ受体3/免疫球蛋白G Fc段受体III抗体
别名	FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.
	Specific References (12) \| bs-6028R has been referenced in 12 publications. [IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635 WB, IF, FC ; Mouse. PubMed:10.1016/j.biomaterials.2022.121635 [IF=11.492] Laura Lopez-Sanz. et al. Fcγ receptor activation mediates vascular inflammation and abdominal aortic aneurysm development. Clin Transl Med. 2021 Jul;11(7):e463 IHC ; Human. PubMed:34323424 [IF=6.147] Weishi Liang. et al. The Role of Microglia/Macrophages Activation and TLR4/NF-κB/MAPK Pathway in Distraction Spinal Cord Injury-Induced Inflammation. FRONT CELL NEUROSCI. 2022; 16: 926453 IF ; Pig. PubMed:35755773 [IF=5.732] Sun Wei. et al. The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring. CLIN EXP IMMUNOL. 2022 Sep;: IF ; Mouse. PubMed:36149061 [IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640 WB ; Rat. PubMed:37196812 [IF=4.657] He, Xiaojing. et al. Inhibition of PD-1 Alters the SHP1/2-PI3K/Akt Axis to Decrease M1 Polarization of Alveolar Macrophages in Lung Ischemia–Reperfusion Injury. INFLAMMATION. 2022 Nov;:1-16 IHC ; Rat. PubMed:36357527 [IF=4.486] Yang Jiao. et al. Inhibition of microglial receptor‐interacting protein kinase 1 ameliorates neuroinflammation following cerebral ischaemic stroke. J Cell Mol Med. 2020 Nov;24(21):12585-12598 WB,IF ; Mouse. PubMed:32990414 [IF=3.943] Yihan Lu et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563. IF ; Mouse. PubMed:32442899 [IF=3.59] Hanshu Zhao. et al. PHLDA1 Blockade Alleviates Cerebral Ischemia/Reperfusion Injury by Affecting Microglial M1/M2 Polarization and NLRP3 Inflammasome Activation. Neuroscience. 2022 Jan;: IF ; Mouse. PubMed:35093445 [IF=3.37] Jing Liet al. hUC-MSCs ameliorated CUMS-induced depression by modulating complement C3 signaling-mediated microglial polarization during astrocyte-microglia crosstalk. Brain Res Bull . 2020 Oct;163:109-119. IHC ; mouse. PubMed:32681971 [IF=3.041] An G et al. Effects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor‑associated macrophages. Oncol Rep. 2019 Dec;42(6):2499-2511. IHC-P ; Human. PubMed:31578575 [IF=2.38] Ding, Peng, et al. "Expression of Tumor-Associated Macrophage in Progression of Human Glioma." Cell Biochemistry and Biophysics (2014): 1-7. IHC-P ; Human. PubMed:25182001
研究领域	细胞生物免疫学干细胞细胞表面分子细胞类型标志物
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Pig,Cow,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	27kDa
细胞定位	细胞膜分泌型蛋白
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human IGFR3/CD16: 131-230/254 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] Function: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Subunit: Exists as a heterooligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1. Subcellular Location: Cell membrane. Secreted. Exists also as a soluble receptor. Tissue Specificity: Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. Post-translational modifications: Glycosylated. Contains high mannose- and complex-type oligosaccharides. The soluble form is produced by a proteolytic cleavage. [MISCELLANEOUS] Encoded by one of two nearly indentical genes: FCGR3A (Shown here) and FCGR3B which are expressed in a tissue-specific manner. The Phe-203 in III-A determines the transmembrane domains whereas the 'Ser-203' in III-B determines the GPI-anchoring. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P08637 Gene ID: 2214 Database links: Entrez Gene: 2214 Human Entrez Gene: 14131 Mouse Entrez Gene: 304966 Rat Omim: 146740 Human SwissProt: P08637 Human SwissProt: P08508 Mouse SwissProt: P27645 Rat Unigene: 372679 Human
产品图片	Sample: Lane 1: Thymus (Rat) Lysate at 40 ug Lane 2: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD16 (bs-6028R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kD Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- CD16 (bs-6028R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD16) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Cell: U937(4% Paraformaldehyde fixed for 10 minutes,2% BSA at 4∑blocked for 30 minutes.). Concentration:1:100;Incubation: 40 minutes. Flow cytometric analysis of Rabbit Anti-CD16 antibody (bs-6028R)(green) compared with control in the absence of primary antibody (blue) followed by U937. Secondary antibody: Goat Anti-rabbit IgG/PE antibody (bs-0295G-PE)

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记