| 产品编号 | bs-6133R |
| 英文名称 | WNT2 |
| 中文名称 | 信号通路Wnt2抗体 |
| 别 名 | Int 1 related protein; IRP protein; WNT 2; INT1L 1; INT1L1; ONCOGENE INT1 LIKE 1; Wingless type MMTV integration site family member 2; WNT2_HUMAN. |
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Specific References (2) | bs-6133R has been referenced in 2 publications.
[IF=7.666] Bohao Zhao. et al. lncRNA2919 Suppresses Rabbit Dermal Papilla Cell Proliferation via trans-Regulatory Actions. CELLS-BASEL. 2022 Jan;11(15):2443 WB ; Rabbit.
[IF=3.71] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995 WB ; Dog.
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| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT2: 221-320/360 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Subcellular Location: Secreted. Tissue Specificity: Expressed in brain in the thalamus, in fetal and adult lung and in placenta. Similarity: Belongs to the Wnt family. SWISS: P09544 Gene ID: 7472 Database links: Entrez Gene: 7472 Human Entrez Gene: 22413 Mouse Omim: 147870 Human SwissProt: P09544 Human SwissProt: P21552 Mouse Unigene: 567356 Human Unigene: 33653 Mouse |
| 产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- WNT2 (bs-6133R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 38 kD 
Sample:
Lane 1: Human HeLa cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human Jurkat cell lysates Primary: Anti-WNT2 (bs-6133R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
