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Rabbit Anti-RAB9  antibody (bs-6177R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-6177R
英文名称 RAB9
中文名称 ras癌基因家族Rab9蛋白抗体
别    名 DmRab9; Rab 9; RAB 9A; RAB9 member RAS oncogene family; RAB9A; RAB9A member RAS oncogene family; RAS ASSOCIATED PROTEIN RAB9; Ras related protein Rab 9A; Sid6061p; Sid99; RAB9A_HUMAN.  
研究领域 肿瘤  细胞生物  免疫学  信号转导  G蛋白偶联受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Chicken,Dog,Pig,Cow,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 23kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RAB9: 65-170/201 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Endoplasmic reticulum membrane (Potential). Golgi apparatus membrane (Potential). Late endosome.

Similarity:
Belongs to the small GTPase superfamily. Rab family.

SWISS:
P51151

Gene ID:
9367

Database links:

Entrez Gene: 511776 Cow

Entrez Gene: 403947 Dog

Entrez Gene: 9367 Human

Entrez Gene: 56382 Mouse

Entrez Gene: 84589 Rat

Omim: 300284 Human

SwissProt: P24408 Dog

SwissProt: P51151 Human

SwissProt: Q9R0M6 Mouse

SwissProt: Q99P75 Rat

Unigene: 495704 Human

Unigene: 728400 Human

Unigene: 25306 Mouse

Unigene: 35289 Rat



产品图片
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-RAB9 (bs-6177R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-RAB9 (bs-6177R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-RAB9 (bs-6177R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RAB9 Polyclonal Antibody, Unconjugated(bs-6177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB9) Polyclonal Antibody, Unconjugated (bs-6177R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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