| 产品编号 | bs-6204R |
| 英文名称 | NCKAP1 |
| 中文名称 | 膜相关的蛋白质HEM2抗体 |
| 别 名 | NCKP1_HUMAN; Nck-associated protein 1; NAP 1; Membrane-associated protein HEM-2; p125Nap1; HEM2; KIAA0587; NAP1; NAP125; |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 干细胞 细胞凋亡 转录调节因子 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Pig,Cow,Horse,GuineaPig) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 129kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCKAP1: 1021-1128/1128 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Apr 2022] Function: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Subcellular Location: Cell membrane. Cell projection Tissue Specificity: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter Similarity: Belongs to the HEM-1/HEM-2 family. SWISS: Q9Y2A7 Gene ID: 10787 Database links: Entrez Gene: 10787 Human Entrez Gene: 50884 Mouse SwissProt: Q9Y2A7 Human SwissProt: P28660 Mouse |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human 293T cell lysates Primary: Anti-NCKAP1 (bs-6204R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kDa Observed band size: 135 kDa 
Sample:
Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti- NCKAP1 (bs-6204R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kD Observed band size: 129 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
