| 产品编号 | bs-6213R |
| 英文名称 | Estrogen Related Receptor gamma Rabbit pAb |
| 中文名称 | 雌激素受体相关蛋白3抗体 |
| 别 名 | ERR 3; ERR G2; ERR gamma 2; ERR gamma-2; ERR3; ERR3_HUMAN; ERRG 2; ERRG2; ESRRG; Estrogen receptor related protein 3; Estrogen receptor-related protein 3; Estrogen-related receptor gamma; NR3B3; Nuclear receptor subfamily 3 group B member 3. |
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Specific References (1) | bs-6213R has been referenced in 1 publications.
[IF=3.258] Liu H et al. Cybrid Model Supports Mitochondrial Genetic Effect on Pig Litter SizeFront Genet.2020 Dec 15;11:579382. WB ; pig.
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| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ESRRG/Estrogen Related Receptor gamma: 61-160/458 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region. Function: Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements Subunit: Homodimer. Binds TLE1, PNRC1 and PNRC2. Binds GRIP1 (By similarity). Interacts with NRIP1, NCOA1 and NCOR2. Subcellular Location: Nucleus Tissue Specificity: Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland. Post-translational modifications: Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity. Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity. Similarity: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P62508 Gene ID: 2104 Database links: Entrez Gene: 2104 Human Entrez Gene: 26381 Mouse Entrez Gene: 405890 Zebrafish Omim: 602969 Human SwissProt: P62508 Human SwissProt: P62509 Mouse Unigene: 444225 Human Unigene: 388156 Mouse Unigene: 89989 Mouse Unigene: 214491 Rat |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Estrogen Related Receptor gamma (bs-6213R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Estrogen Related Receptor gamma (bs-6213R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Estrogen Related Receptor gamma (bs-6213R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
