产品编号 | bs-6236R |
英文名称 | OTUB2 |
中文名称 | 泛素特异性蛋白酶Otubain2抗体 |
别 名 | C14orf137; Deubiquitinating enzyme OTUB2; OTB2; OTU domain containing ubiquitin aldehyde binding protein 2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; OTUB2_HUMAN; Otubain 2; Otubain-2; Ubiquitin specific processing protease OTUB2; Ubiquitin specific protease otubain 2; Ubiquitin thioesterase OTUB2; Ubiquitin-specific-processing protease OTUB2. |
Specific References (1) | bs-6236R has been referenced in 1 publications.
[IF=7.717] Liu, Li. et al. OTUB2 Regulates YAP1/TAZ to Promotes the Progression of Esophageal Squamous Cell Carcinoma. BIOL PROCED ONLINE. 2022 Dec;24(1):1-14 IHC ; Human.
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研究领域 | 细胞生物 信号转导 细胞周期蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse (predicted: Human,Rat,Dog,Pig,Cow,Rabbit,Sheep) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 27kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human OTUB: 21-110/234 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Tissue Specificity: Widely expressed. Expressed at higher level in brain. Similarity: Belongs to the peptidase C65 family. Contains 1 OTU domain. SWISS: Q96DC9 Gene ID: 78990 Database links: Entrez Gene: 78990 Human Entrez Gene: 68149 Mouse Omim: 608338 Human SwissProt: Q96DC9 Human SwissProt: Q9CQX0 Mouse Unigene: 278815 Human Unigene: 127378 Mouse Unigene: 18862 Rat |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUB2) Polyclonal Antibody, Unconjugated (bs-6236R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |