| 产品编号 | bs-6281R |
| 英文名称 | Hepsin/TMPRSS1 |
| 中文名称 | 跨膜蛋白酶丝氨酸1抗体 |
| 别 名 | HEPS; HEPS_HUMAN; Hepsin; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non catalytic chain; TMPRSS 1; TMPRSS1;TMPRSS-1;Transmembrane protease serine 1. |
| 研究领域 | 肿瘤 细胞生物 信号转导 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hepsin: 311-417/417 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants. Function: Plays an essential role in cell growth and maintenance of cell morphology. May mediate the activating cleavage of HGF and MST1/HGFL. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Present in most tissues, with the highest level in liver. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 1 SRCR domain. SWISS: P05981 Gene ID: 3249 Database links: Entrez Gene: 3249 Human Omim: 142440 Human SwissProt: P05981 Human Unigene: 182385 Human |
| 产品图片 |
Sample: HepG2 (human)Cell Lysate at 40 ug
Primary: Anti- Hepsin/TMPRSS1 (bs-6281R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 28 kD 
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Hepsin Polyclonal Antibody, Unconjugated(bs-6281R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
