| 产品编号 | bs-6621R |
| 英文名称 | CHX10 Rabbit pAb |
| 中文名称 | CHX10蛋白抗体 |
| 别 名 | C elegans ceh 10 homeo domain containing homolog; Ceh 10 homeo domain containing homolog(C. elegans); Ceh 10 homeo domain containing homolog; Ceh 10 homeodomain containing homolog; Ceh 10 homeodomain containing homolog(C. elegans); Ceh10 homeo domain containing homolog; Ceh10 homeodomain containing homolog; CHX 10; Homeobox protein CHX 10; Homeobox protein CHX10; HOX 10; Visual system homeobox 2; HOX10; MCOP 2; MCOP2; MCOPCB 3; MCOPCB3; RET 1; RET1; Vsx 2; Vsx2. |
| 研究领域 | 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHX10: 251-361/361 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CHX10 is a 40kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in CHX10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina CHX10 is expressed in retinal progenitors, while in the mature retina, CHX10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the CHX10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. CHX10 is also expressed in the developing brainstem, thalamus, and spinal cord. Function: Plays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate. DISEASE: Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present. Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092]. Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Similarity: Belongs to the paired homeobox family. Contains 1 CVC domain. Contains 1 homeobox DNA-binding domain. SWISS: P58304 Gene ID: 338917 Database links: Entrez Gene: 338917 Human Entrez Gene: 12677 Mouse Omim: 142993 Human SwissProt: P58304 Human SwissProt: Q61412 Mouse Unigene: 449771 Human Unigene: 4405 Mouse Unigene: 92414 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Eye tissue lysates
Lane 2: Human Raji cell lysates
Lane 3: Human Ramos cell lysates
Primary: Anti-CHX10 (bs-6621R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 35 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
