| 产品编号 | bs-6626R |
| 英文名称 | GPR24 Rabbit pAb |
| 中文名称 | G蛋白偶联受体24抗体 |
| 别 名 | MCHR; G protein coupled receptor 24; GPCR MCH1; GPCR SLC/MCH1; GPR24; MCH receptor 1; MCH1R; Melanin concentrating hormone receptor 1; SLC-1; Somatostatin receptor like protein; MCHR1_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCHR/GPR24: 75-180/422 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MCHR, a member of the G protein coupled receptor family 1, is an integral plasma membrane protein which binds melanin concentrating hormone. MCHR can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, MCHR does not seem to bind somatostatin. Function: Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis. Subunit: Interacts with NCDN. Subcellular Location: Cell Membrane; multi-pass membrane protein Tissue Specificity: Highest level in brain, particularly in the frontal cortex and hypothalamus, lower levels in the liver and heart. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q99705 Gene ID: 2847 Database links: Entrez Gene: 2847 Human Entrez Gene: 207911 Mouse Omim: 601751 Human SwissProt: Q99705 Human SwissProt: Q8JZL2 Mouse Unigene: 248122 Human Unigene: 323523 Mouse Unigene: 10822 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR24 Polyclonal Antibody, Unconjugated(bs-6626R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
