| 产品编号 | bs-6645R |
| 英文名称 | WNT7A |
| 中文名称 | 原癌基因wnt7a蛋白抗体 |
| 别 名 | Protein Wnt-7a; wnt 7a;Protein Wnt-7a precursor; proto-oncogene wnt7a protein; wingless-type MMTV integration site family, member 7A; WNT7A; WNT7A_HUMAN. |
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Specific References (2) | bs-6645R has been referenced in 2 publications.
[IF=5.738] Jianglin Wang. et al. Validation and analysis of expression, prognosis and immune infiltration of WNT gene family in non-small cell lung cancer. FRONT ONCOL. 2022; 12: 911316 WB, IHC ; Human.
[IF=0.375] ZHANG P et al. 5-Azacytidine and trichostatin A enhance the osteogenic differentiation of bone marrow mesenchymal stem cells isolated from steroid-induced avascular necrosis of the femoral head in rabbit. J Biosci (2019) 44:87. WB ; Rabbit.
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| 研究领域 | 肿瘤 信号转导 干细胞 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT7A: 241-349/349 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts (By similarity). Subunit: Interacts with PORCN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. DISEASE: Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS) [MIM:276820]. A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Similarity: Belongs to the Wnt family. SWISS: O00755 Gene ID: 7476 Database links: Entrez Gene: 7476 Human Entrez Gene: 100055450 Horse Entrez Gene: 22421 Mouse Entrez Gene: 100355697 Rabbit Omim: 601570 Human SwissProt: O00755 Human SwissProt: P24383 Mouse Unigene: 72290 Human Unigene: 56964 Mouse |
| 产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-WNT7A (bs-6645R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD 
Sample:
Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Mouse Testis tissue lysates Lane 4: Mouse Kidney tissue lysates Lane 5: Rat Placenta tissue lysates Lane 6: Rat Lung tissue lysates Lane 7: Rat Testis tissue lysates Lane 8: Rat Kidney tissue lysates Lane 9: Human Hela cell lysates Lane 10: Human A431 cell lysates Lane 11: Human SH-SY5Y cell lysates Lane 12: Human HepG2 cell lysates Primary: Anti- WNT7A (bs-6645R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
