产品编号 | bs-6690R |
英文名称 | Transglutaminase-1 Rabbit pAb |
中文名称 | 谷氨酰胺转胺酶1抗体 |
别 名 | TGM1_HUMAN; Epidermal TGase; ICR 2; ICR2; K polypeptide epidermal type I; KTG; LI 1; LI; LI1; Protein glutamine gamma glutamyltransferase K; TG(K); TGase 1; TGASE; TGase K; TGase1; TGaseK; TGK; Tgm 1; Tgm1 antibody Transglutaminase 1; Transglutaminase 1; Transglutaminase K; Transglutaminase keratinocyte; Transglutaminase1; TransglutaminaseK. |
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Specific References (1) | bs-6690R has been referenced in 1 publications.
[IF=2.3] Xie, Xin, et al. "Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes."Biochemical and Biophysical Research Communications (2016). WB ; Human.
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研究领域 | 信号转导 结合蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Rat (predicted: Mouse,Pig,Dog) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 90 kDa |
检测分子量 | |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Transglutaminase-1: 461-560/817 |
亚 型 | |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Tissue transglutaminase is a calcium activated enzyme which catalyzes the cross linking of proteins and the conjugation of polyamines to proteins. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD). CD, a genetic, immunologically mediated small bowel enteropathy that causes malabsorption, is a relatively common disorder in Western countries. Function: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Subcellular Location: Membrane; Lipid-anchor. Similarity: Belongs to the transglutaminase superfamily. SWISS: P22735 Gene ID: 7051 Database links: Entrez Gene: 7051 Human Omim: 190195 Human SwissProt: P22735 Human Unigene: 508950 Human |
产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Transglutaminase Polyclonal Antibody, Unconjugated(bs-6690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Transglutaminase antibody (bs-6690R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |