| 产品编号 | bs-7022R |
| 英文名称 | IFNAR2 |
| 中文名称 | 干扰素α受体2抗体 |
| 别 名 | IFN alpha REC; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-R-2; IFNABR; IFNAR2; IFNARB; IFNR; INAR2_MOUSE; Interferon alpha binding protein; Interferon alpha/beta receptor 2; Interferon alpha/beta receptor beta chain; Type I interferon receptor 2; Type I interferon receptor. |
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Specific References (2) | bs-7022R has been referenced in 2 publications.
[IF=2.66] Lopušná, Katarína, et al. "Murine gammaherpesvirus targets type I IFN receptor but not type III IFN receptor early in infection." Cytokine 83 (2016): 158-170. WB ; Mouse.
[IF=1.5] Sakumoto R et al. Pregnancy-associated changes of Peroxisome Proliferator-Activated Receptor Delta (PPARD) and Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) expression in the bovine corpus luteum. J Reprod Dev. 2020 Feb 7. IHC-P ; Bovine.
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| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 转录调节因子 表观遗传学 干扰素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse IFNAR2: 165-260/513 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 2 and 3 may be potent inhibitors of type I IFN receptor activity. Subunit: Heterodimer with IFNAR1; in presence of interferon alpha and/or beta ligands forms the type I interferon receptor. Isoform 1 interacts with the transcriptional factors STAT1 and STAT2. Interacts with JAK1. Subcellular Location: Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Secreted (Probable). Isoform 3: Secreted (Probable). Tissue Specificity: Widely expressed. Detected in liver, testis, kidney, salivary gland, thymus, brain, lung and placenta. Isoform 1, isoform 2 and isoform 3 are expressed in brain. Post-translational modifications: Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-335 or Tyr-510 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types. Glycosylated. Similarity: Belongs to the type II cytokine receptor family. SWISS: O35664 Gene ID: 15976 Database links: Entrez Gene: 15976 Mouse |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37閳�?? for 20 min; Incubation: Anti-IFNAR2/IFNABR Polyclonal Antibody, Unconjugated(bs-7022R) 1:200, overnight at 4鎺矯, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
