| 产品编号 | bs-7034R |
| 英文名称 | EphA7 |
| 中文名称 | 酪氨酸蛋白激酶受体A7抗体 |
| 别 名 | Cek 11; Developmental kinase 1; EBK; EHK-3; EHK3; EK11; Embryonic brain kinase; Eph homology kinase 3; Eph receptor A7; EPH-like kinase 11; EphA7; EPHA7_HUMAN; Ephrin receptor Eph A7; Ephrin type A receptor 7; Ephrin type-A receptor 7; hEK11; MDK 1; Receptor protein tyrosine kinase HEK 11; Tyrosine protein kinase receptor EHK 3. |
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Specific References (1) | bs-7034R has been referenced in 1 publications.
[IF=7.561] Alexandru Tatomir. et al. RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis. Front Immunol. 2021; 12: 705308 IHC ; Mouse.
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| 研究领域 | 心血管 细胞生物 免疫学 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50/107kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EphA7: 181-280/998 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). Receptor for members of the ephrin-A family. Binds to ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4 and ephrin-A5. Function: ’Receptor tyrosine kinase which binds promiscuouslyGPI-anchored ephrin-A family ligands residing on adjacent cells,leading to contact-dependent bidirectional signaling intoneighboring cells. The signaling pathway downstream of the receptoris referred to as forward signaling while the signaling pathwaydownstream of the ephrin ligand is referred to as reversesignaling. Among GPI-anchored ephrin-A ligands, EFNA5 is acognate/functional ligand for EPHA7 and their interaction regulatesbrain development modulating cell-cell adhesion and repulsion. Hasa repellent activity on axons and is for instance involved in theguidance of corticothalamic axons and in the proper topographicmapping of retinal axons to the colliculus. May also regulate braindevelopment through a caspase(CASP3)-dependent proapoptoticactivity. Forward signaling may result in activation of componentsof the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 ANDMAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4which lacks the kinase domain may regulate isoform 1 adhesiveproperties. Subunit: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain). Subcellular Location: Cell membrane (Probable); Single-pass type I membrane protein (Probable). Tissue Specificity: Widely expressed in embryo. In adult, expression restricted to hippocampus, testis and spleen. Post-translational modifications: Phosphorylated. Isoform 4 inhibits isoform 1 phosphorylationand may regulate its function in adhesion. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 Eph LBD (Eph ligand-binding) domain. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q15375 Gene ID: 2045 Database links: Entrez Gene: 2045 Human Entrez Gene: 13841 Mouse Omim: 602190 Human SwissProt: Q15375 Human SwissProt: Q61772 Mouse Unigene: 73962 Human Unigene: 257266 Mouse Unigene: 10181 Rat |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug Primary: Anti-EphA7 (bs-7034R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kD 
Protein: rat brain lysates, 30ug;
Primary: Anti-EphA7 (bs-7034R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 107kD Observed band size : 45kD 
Sample:
Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-EphA7 (bs-7034R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/107 kD Observed band size: 52 kD 
Tissue/cell: rat brain tissue(hippocampus); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphA7 Polyclonal Antibody, Unconjugated(bs-7034R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
