| 产品编号 | bs-7518R |
| 英文名称 | Ribonuclease Inhibitor Rabbit pAb |
| 中文名称 | 核糖核酸酶抑制因子1抗体 |
| 别 名 | Placental ribonuclease inhibitor; RNH; RNH1; Placental RNase inhibitor; PRI; RAI; RI antibody Ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; Ribonuclease/angiogenin inhibitor; RINI_HUMAN; RNase inhibitor; RNH 1; Rnh1 ribonuclease/angiogenin inhibitor 1. |
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Specific References (1) | bs-7518R has been referenced in 1 publications.
[IF=1.6] Tang, Y., et al. "Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway." Clinical and Translational Oncology: 1-8. IHC-P ; Human.
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| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ribonuclease Inhibitor: 101-200/461 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis. Subunit: Forms high-affinity heterodimers with RNASE1, ANG and RNASE2. Subcellular Location: Cytoplasm. Post-translational modifications: The N-terminus is blocked. At least 30 of the 32 cysteine residues are in the reduced form. Similarity: Contains 15 LRR (leucine-rich) repeats. SWISS: P13489 Gene ID: 6050 Database links: Entrez Gene: 6050 Human Entrez Gene: 107702 Mouse Omim: 173320 Human SwissProt: P13489 Human SwissProt: Q91VI7 Mouse Unigene: 530687 Human
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| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control(blue): Hela cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Ribonuclease Inhibitor antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
