| 产品编号 | bs-7057R |
| 英文名称 | Glucokinase regulatory protein Rabbit pAb |
| 中文名称 | 葡萄糖激酶调节蛋白抗体 |
| 别 名 | FGQTL5; GCKR; GCKR_HUMAN; GKRP; GLRE; glucokinase(hexokinase 4) regulator; Glucokinase regulator; Glucokinase regulatory protein; OTTHUMP00000158533. |
| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 转录调节因子 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCKR: 121-220/625 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Inhibits glucokinase by forming an inactive complex with this enzyme. Tissue Specificity: Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. Similarity: Belongs to the GCKR family. Contains 2 SIS domains. SWISS: Q14397 Gene ID: 2646 Database links: Entrez Gene: 2646 Human Entrez Gene: 231103 Mouse Omim: 600842 Human SwissProt: Q14397 Human SwissProt: Q91X44 Mouse Unigene: 89771 Human Unigene: 100043 Mouse |
| 产品图片 |
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GCKR/Glucokinase regulatory protein Polyclonal Antibody, Unconjugated (bs-7057R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
