[IF=5.279] Lei Wang. et al. Lactobacillus plantarum DP189 Reduces α-SYN Aggravation in MPTP-Induced Parkinson’s Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):1163–1173  WB ;  Mouse.  

PubMed:35067061

[IF=5.26] Meng, Guoliang, et al. "Hydrogen sulfide pretreatment improves mitochondrial function in myocardial hypertrophy via a SIRT3 dependent manner." British Journal of Pharmacology (2017).  WB ;  Mouse.  

PubMed:28503736

[IF=4.315] Ma, Dufang. et al. Astragalus polysaccharide prevents heart failure-induced cachexia by alleviating excessive adipose expenditure in white and brown adipose tissue. LIPIDS HEALTH DIS. 2023 Dec;22(1):1-16  WB ;  Rat.  

PubMed:36670439

[IF=3.776] Tingting Zhao. et al. Yishen Huashi granule modulated lipid metabolism in diabetic nephropathy via PI3K/AKT/mTOR signaling pathways.. HELIYON. 2023 Mar;9(3):e14171-e14171  WB ;  Human.  

PubMed:36938470

[IF=3.189] Tingting Li. et al. Cold exposure induces browning of bovine subcutaneous white fat in vivo and in vitro. J THERM BIOL. 2023 Feb;112:103446  WB ;  Bovine.  

PubMed:10.1016/j.jtherbio.2022.103446

[IF=2.952] Ji Hye Hwang. et al. Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway. MOL MED REP. Mol Med Rep. 2022 Jun;25(6):1-10  WB ;  Mouse.  

PubMed:35419614

Sample:
Muscle (Pig) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-PGC1 alpha + beta (bs-4064R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 88/113 kD
Observed band size: 92 kD

Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88/113 kD
Observed band size: 113 kD

Sample:
Lane 1: Molt-4 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90/113 kD
Observed band size: 113 kD

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (bs-7535R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-PGC1 alpha+beta antibody (bs-7535R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.