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Rabbit Anti-TNF alpha antibody (bs-2150R)
~~~促销,代码SSY221101~~~
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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-2150R
英文名称 TNF alpha
中文名称 肿瘤坏死因子-α抗体
别    名 Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha; TNFA_HUMAN.  
Specific References  (12)     |     bs-2150R has been referenced in 12 publications.
[IF=3.738] Zhou, Can-Can. et al. Lead Exposure in Developmental Ages Promotes Aβ Accumulation by Disturbing Aβ Transportation in Blood-Cerebrospinal Fluid Barrier/Blood–Brain Barriers and Impairing Aβ Clearance in the Liver. 2021 Nov 17  IHC ;  Rat.  
[IF=2.633] Claudia C. Cotomacio. et al. Wound healing process with different photobiomodulation therapy protocols to treat 5-FU-induced oral mucositis in hamsters. Arch Oral Biol. 2021 Nov;131:105250  IHC ;  Syrian Hamster.  
[IF=6.832] Yanhong Li. et al. Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model. Stem Cell Res Ther. 2021 Dec;12(1):1-14  IHC ;  Rabbit.  
[IF=3.69] Da Zhao. et al. Exploring the Regulatory Mechanism of Nao Tai Fang on Vascular Dementia's Biological Network Based on Cheminformatics and Transcriptomics Strategy. J Ethnopharmacol. 2021 Mar;:114065  IHC ;  Rat.  
[IF=3.17] Meng H et al. Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis. Eur J Pharmacol. 2019 Nov 5;862:172642.  WB ;  Mouse.  
[IF=3.28] Liao, Peng, et al. "Eccentric contraction induces inflammatory responses in rat skeletal muscle: role of tumor necrosis factor-α." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 298.3 (2010): R599-R607.  WB ;  Rat.  
[IF=6.38] Zhang, Jing-Jing, et al. "“Proof-of-principle” concept for ultrasensitive detection of cytokines based on the electrically heated carbon paste electrode." Chemical Communications 47.23 (2011): 6551-6553.  
[IF=3.569] Guoxiu Wang. et al. Local renal complement activation mediates immune kidney injury by inducing endothelin-1 signalling and inflammation in trichloroethylene-sensitised mice. Toxicol Lett. 2020 Oct;333:130  IHC ;  Mouse.  
[IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.  WB ;  Rat.  
[IF=3.457] Hu Y et al.Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors.(2018) Biomedicine & Pharmacotherapy, 108, 1294–1302.  IHC,WB ;  Rat.  
[IF=3.514] Zhang J et al. Role of selective blocking of bradykinin B1 receptor in attenuating immune liver injury in trichloroethylene-sensitized mice. Cytokine. 2018 Aug;108:71-81.  IHC-P ;  Mouse.  
[IF=2.31] Xi et al. Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii). (2017) Eur.J.Histochem. 61:2834  IHC-P ;  Frog.  
研究领域 肿瘤  细胞生物  信号转导  细胞凋亡  生长因子和激素  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,  (predicted: Dog, Pig, Rabbit, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 17/26kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TNF-alpha: 181-235/233 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]

Function:
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subunit:
Homotrimer. Interacts with SPPL2B.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.
Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein.
Tumor necrosis factor, soluble form: Secreted.
C-domain 1: Secreted.
C-domain 2: Secreted.

Post-translational modifications:
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

DISEASE:
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
P01375

Gene ID:
7124

Database links:

Entrez Gene: 7124 Human

Entrez Gene: 21926 Mouse

Omim: 191160 Human

SwissProt: P01375 Human

SwissProt: P06804 Mouse

Unigene: 241570 Human

Unigene: 1293 Mouse





产品图片
Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-2150R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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