| 产品编号 | bs-7059R |
| 英文名称 | MMP9 |
| 中文名称 | 基质金属蛋白酶9抗体 |
| 别 名 | Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV. Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN. |
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Specific References (2) | bs-7059R has been referenced in 2 publications.
[IF=3.457] Gao H et al.Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway.(2018) Biomed. Pharmacother 110 WB ; Mouse.
[IF=0] Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38 IHC ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Dog,Pig,Horse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 66/76kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP-9: 371-470/707 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]. Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Subunit: Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Tissue Specificity: Produced by normal alveolar macrophages and granulocytes. Post-translational modifications: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. DISEASE: Intervertebral disc disease (IDD) [MIM:603932]: A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Metaphyseal anadysplasia 2 (MANDP2) [MIM:613073]: A bone development disorder characterized by skeletal anomalies that resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin repeats. SWISS: P14780 Gene ID: 4318 Database links: Entrez Gene: 4318 Human Entrez Gene: 17395 Mouse Omim: 120361 Human SwissProt: P14780 Human SwissProt: P41245 Mouse Unigene: 297413 Human Unigene: 4406 Mouse Unigene: 10209 Rat MMP9亦称IV型胶原酶或明胶酶B，其主要功能为降解IV型胶原。因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。 目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与转移浸润的研究。细胞外基质在维持正常组织结构与功能以及细胞生长和分化过程中起重要作用。细胞外基质动态平衡的失调与肿瘤细胞侵袭、转移和复发密切相关，基质金属蛋白酶(MMP9)是细胞外基质的降解酶，可降解Ⅳ、Ⅴ、Ⅸ、Ⅺ型胶原，在肿瘤的浸润、转移过程中起重要作用，近年为肿瘤研究的热点。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
