| 产品编号 | bs-2976R |
| 英文名称 | GGT1 |
| 中文名称 | γ谷氨酰转移酶抗体 |
| 别 名 | CD224; Gamma glutamyl transpeptidase; Gamma glutamyltransferase 1; Gamma glutamyltranspeptidase 1; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1 light chain; GGT 1; GGT; GGT1; GGT1_HUMAN; Glutamyl transpeptidase; GTG. |
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Specific References (1) | bs-2976R has been referenced in 1 publications.
[IF=2.52] Kobayashi S et al. γ-Glutamylcysteine synthetase and γ-glutamyl transferase as differential enzymatic sources of γ-glutamylpeptides in mice. Amino Acids. 2020 Apr;52(4):555-566. WB ; mouse.
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| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GGT1: 351-460/569 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Tissue specificity: Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. Subunit: Heterodimer composed of the light and heavy chains. The active site is located in the light chain. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. Similarity: Belongs to the gamma-glutamyltransferase family. SWISS: P19440 Gene ID: 2678 Database links: Entrez Gene: 2678 Human Entrez Gene: 14598 Mouse Omim: 612346 Human SwissProt: P19440 Human SwissProt: Q60928 Mouse Unigene: 595809 Human Unigene: 645535 Human Unigene: 4559 Mouse Unigene: 10010 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse kidney lysates Lane 2: Mouse Stomach lysates Lane 3: Mouse Large intestine lysates Primary: Anti-GGT1 (bs-2976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa 
Sample:
Lane 1: Liver (Mouse) Tissue Lysate at 40 ug Lane 2: Liver (Rat) Tissue Lysate at 40 ug Lane 3: Thymus (Rat) Tissue Lysate at 40 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Lane 5: U937 (Human) Cell Lysate at 30 ug Lane 6: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-GGT1 (bs-2976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD 
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-GGT1 (bs-2976R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
