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Rabbit Anti-IL-1 Beta antibody (bs-6319R)
~~~促销,代码SSY221101~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-6319R
英文名称 IL-1 Beta
中文名称 白介素1β抗体
别    名 IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta; IL1F2; IL-1 beta; Catabolin; IL-1; IL1F2; IL1beta; IL1-BETA; IL-1beta; IL-1 Beta; IL-1β; IL1β; IL-1B; IL1B;   
Specific References  (23)     |     bs-6319R has been referenced in 23 publications.
[IF=3.895] Yang W et al. The Effect of Low and High Dose Deoxynivalenol on Intestinal Morphology, Distribution, and Expression of Inflammatory Cytokines of Weaning Rabbits. Toxins (Basel). 2019 Aug 13;11(8). pii: E473.  WB&IHC-P ;  Rabbit.  
[IF=2.882] Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099  IHC ;  Rat.  
[IF=11.799] Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134  WB ;  mosue.  
[IF=3.412] Abdelaziz Adam Idriss Arbab. et al. Metformin Inhibits Lipoteichoic Acid–Induced Oxidative Stress and Inflammation Through AMPK/NRF2/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells. Front Vet Sci. 2021; 8: 661380  WB ;  Bovine.  
[IF=1.25] Mehmet Balbaba. et al. Anti-inflammatory effect of cortistatin in rat endotoxin-induced uveitis model. Indian J Ophthalmol. 2020 Sep; 68(9): 1920–1924  IHC ;  Rat.  
[IF=7.238] Chinthalapally V. Rao. et al. GSK3‐ARC/Arg3.1 and GSK3‐Wnt signaling axes trigger amyloid‐β accumulation and neuroinflammation in middle‐aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221  IHC ;  Mouse.  
[IF=3.69] Dai-Chao Ma. et al. Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro. J Ethnopharmacol. 2021 Apr;270:113776  WB ;  Rat.  
[IF=0] Yamada H et al. LATE-ONSET ALZHEIMER'S DISEASE ANIMAL MODEL AND USES THEREOF. US Patent20190387723  WB, IHC-P&IHF ;  Mouse.  
[IF=4.13] Fukuda M et al. Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation. J Neurosurg. 2019 Dec 20:1-13.  WB ;  Mouse.  
[IF=3.17] Yu H et al. Echinocystic acid, a natural plant extract, alleviates cerebral ischemia/reperfusion injury via inhibiting the JNK signaling pathway. Eur J Pharmacol. 2019 Oct 15;861:172610.  WB ;  Mouse.  
[IF=3.895] Sun D et al. Canonical Transient Receptor Potential Channel 3 Contributes to Febrile Seizure Inducing Neuronal Cell Death and Neuroinflammation.Cell. Mol. Neurobiol. 2018 Aug;38(6), 1215–1226.  IF ;  Rat.  
[IF=2.55] Dinesh, Palani, and MahaboobKhan Rasool. "Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats." International Immunopharmacology 44 (2017): 26-37.  WB ;  Mouse.  
[IF=3.921] Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-κB signaling pathway. Neurochem Int. 2021 Nov;:105221  WB ;  Rat.  
[IF=2.695] Jaclyn Iannucci. et al. Isoform-Specific Effects of Apolipoprotein E on Markers of Inflammation and Toxicity in Brain Glia and Neuronal Cells In Vitro. Curr Issues Mol Biol. 2021 Jun;43(1):215-225  WB ;  Human.  
[IF=4.858] Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163–3173  WB ;  Rat, Human.  
[IF=4.966] Wei‐Cheng Chen. et al. Resistin enhances IL‐1β and TNF‐α expression in human osteoarthritis synovial fibroblasts by inhibiting miR‐149 expression via the MEK and ERK pathways. Faseb J. 2020 Oct;34(10):13671-13684  WB,IHC ;  Human.  
[IF=2.985] Mengmeng Yang. et al. Mesenchymal stem cell-conditioned medium improved mitochondrial function and alleviated inflammation and apoptosis in non-alcoholic fatty liver disease by regulating SIRT1. Biochem Bioph Res Co. 2021 Mar;546:74  WB ;  Mouse, Human.  
[IF=13.116] Zhang X et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer’s diseaseSci Adv.2020 Dec 4;6(49):eabb8680.  IF ;  Mouse.  
[IF=4.691] Dai-Chao Maet al. Kv1.3 channel blockade alleviates cerebral ischemia/reperfusion injury by reshaping M1/M2 phenotypes and compromising the activation of NLRP3 inflammasome in microglia. Exp Neurol . 2020 Oct;332:113399.  WB ;  rat.  
[IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.  WB ;  Rat.  
[IF=1.41] Han JK et al. Anti-inflammatory effect of polydeoxyribonucleotide on zoledronic acid-pretreated and lipopolysaccharide-stimulated RAW 264.7 cells. (2018) Exp.Ther.Med. 16:400-405  WB ;  human.  
[IF=0] Evashwick-Rogler et al. Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model. (2018) JOR.Spine. 1  IHC ;  Rat.  
[IF=2.33] Li, Kang, et al. "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." Molecular Medicine Reports.  WB ;  Human.  
研究领域 细胞生物  免疫学  生长因子和激素  糖蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 17/32kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IL-1 Beta: 161-269/269 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].

Function:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subunit:
Monomer.

Subcellular Location:
Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Similarity:
Belongs to the IL-1 family.

SWISS:
P01584

Gene ID:
3553

Database links:

Entrez Gene: 3553 Human

Entrez Gene: 16176 Mouse

Entrez Gene: 24494 Rat

Omim: 147720 Human

SwissProt: P01584 Human

SwissProt: P10749 Mouse

SwissProt: Q63264 Rat

Unigene: 126256 Human

Unigene: 222830 Mouse

Unigene: 9869 Rat




产品图片
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta (bs-6319R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/30 kD
Observed band size: 30 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta (bs-6319R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 17/30 kD
Observed band size: 30 kD
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- IL-1 Beta (bs-6319R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/30 kD
Observed band size: 30 kD
Sample:
uterus (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta (Bs- 6319R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/30 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-IL-1 Beta antibody (bs-6319R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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