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Rabbit Anti-CIDEC antibody (bs-6796R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-6796R
英文名称 CIDEC
中文名称 细胞死亡活化蛋白抗体
别    名 Cell Death Activator; Cell death activator CIDE-3; Cell Death Inducing DFFA Like Effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDE C; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FSP27.  
研究领域 肿瘤  心血管  细胞生物  免疫学  信号转导  细胞凋亡  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 27kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CIDEC:101-200/238 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010].
Tissue specificity: Expressed mainly in small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver.

Function:
May act as a CEBPB coactivator in white adipose tissueto control the expression of a subset of CEBPB downstream targetgenes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (Bysimilarity). Binds to lipid droplets and regulates theirenlargement, thereby restricting lipolysis and favoring storage. Atfocal contact sites between lipid droplets, promotes directionalnet neutral lipid transfer from the smaller to larger lipiddroplets. The transfer direction may be driven by the internalpressure difference between the contacting lipid droplet pair. Whenoverexpressed in preadipocytes, induces apoptosis or increases cellsusceptibility to apoptosis induced by serum deprivation or TGFBtreatment. As mature adipocytes, that express high CIDEC levels,are quite resistant to apoptotic stimuli, the physiologicalsignificance of its role in apoptosis is unclear.

Subunit:
Interacts with CEBPB (By similarity). Interacts withCIDEA.

Subcellular Location:
Nucleus (By similarity). Endoplasmicreticulum (By similarity). Lipid droplet. Note=Diffuses quickly onlipid droplet surface, but becomes trapped and clustered at lipiddroplet contact sites, thereby enabling its rapid enrichment atlipid droplet contact sites.

Tissue Specificity:
Expressed mainly in adipose tissue, smallintestine, heart, colon and stomach and, at lower levels, in brain,kidney and liver.

Post-translational modifications:
Ubiquitinated and targeted to proteasomal degradation,resulting in a short half-life. Protein stability depends ontriaclyglycerol synthesis, fatty acid availability and lipiddroplet formation (By similarity).

DISEASE:
Note=In omental adipose tissue of obese patients matchedfor BMI, expression levels tend to correlate with insulinsensitivity. Expression is increased 2-3 fold in the group ofpatients with high insulin sensitivity, compared to theinsulin-resistant group. This observation is consistent with theidea that triglyceride storage in adipocytes plays an importantrole in sequestering triglycerides and fatty acids away from thecirculation and peripheral tissues, thus enhancing insulinsensitivity in liver and muscle. This effect is not significant insubcutaneous adipose tissue (PubMed:18509062). In subcutaneousadipose tissue of diabetic patients, tends to negatively correlatewith body mass index and total fat mass, independently of insulinsensitivity (PubMed:18334488).

Similarity:
Contains 1 CIDE-N domain.

SWISS:
Q96AQ7

Gene ID:
63924

Database links:

Entrez Gene: 63924 Human

Entrez Gene: 14311 Mouse

Entrez Gene: 500292 Rat

Omim: 612120 Human

SwissProt: Q96AQ7 Human

SwissProt: P56198 Mouse

SwissProt: Q5XI33 Rat

Unigene: 567562 Human

Unigene: 635072 Human

Unigene: 10026 Mouse

Unigene: 33794 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: Adipose (Mouse) Lysate at 40 ug
Lane 2: Breast (Mouse) Lysate at 40 ug
Lane 3: Adipose (Rat) Lysate at 40 ug
Primary:
Anti-CIDEC (bs-6796R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27-30 kD
Observed band size: 27 kD
Sample:
Epididymis (Mouse) Lysate at 40 ug
Primary: Anti-CIDEC (bs-6796R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (MOUSE colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (bs-6796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(bs-6796R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CIDEC antibody (bs-6796R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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