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Rabbit Anti-CAT  antibody (bs-6874R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  
50ul/1180.00元
100ul/1980.00元
大包装/询价

产品编号 bs-6874R
英文名称 CAT
中文名称 过氧化氢酶抗体
别    名 CATA_HUMAN; Catalase; EC:1.11.1.6.   
Specific References  (9)     |     bs-6874R has been referenced in 9 publications.
[IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635  IF, FC ;  Mouse.  
[IF=8.897] Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-10  WB ;  Mouse.  
[IF=7.675] Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443  IHC ;  Rat.  
[IF=7.468] Dr. Evan Paul Tracy. et al. Stromal Vascular Fraction Restores Vasodilatory Function by Reducing Oxidative Stress in Aging-Induced Coronary Microvascular Disease. ANTIOXID REDOX SIGN. 2022 Aug 11  IF ;  Rat.  
[IF=6.1] Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017).  WB ;  Human.  
[IF=4.868] Wang PW et al. Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties. Oxid Med Cell Longev. 2019 Jan 6;2019:9529676.  WB ;  Human.  
[IF=3.69] Sheng Zhang. et al. Proteomics analysis in the kidney of mice following oral feeding Realgar. J Ethnopharmacol. 2021 Jul;275:114118  WB ;  Mouse.  
[IF=3.47] Minjeong Kim. et al. Ibuprofen Increases the Hepatotoxicity of Ethanol through Potentiating Oxidative Stress. Biomol Ther. 2021 Mar 1; 29(2): 205–210  WB ;  Human.  
[IF=2.323] Liu R et al. Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of LambsAnimals (Basel).2020 Dec 23;11(1):7.  WB ;  Lamb.  
研究领域 肿瘤  细胞生物  免疫学  发育生物学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 60kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Catalase: 251-350/527 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

Function:
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Subunit:
Homotetramer.

Subcellular Location:
Peroxisome.

Post-translational modifications:
The N-terminus is blocked.

DISEASE:
Defects in CAT are the cause of acatalasemia (ACATLAS) [MIM:614097]. A metabolic disorder characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

Similarity:
Belongs to the catalase family.

SWISS:
P04040

Gene ID:
847

Database links:

Entrez Gene: 847 Human

Entrez Gene: 12359 Mouse

Entrez Gene: 24248 Rat

Omim: 115500 Human

SwissProt: P04040 Human

SwissProt: P24270 Mouse

SwissProt: P04762 Rat

Unigene: 502302 Human

Unigene: 4215 Mouse

Unigene: 3001 Rat



过氧化氢酶是催化过氧化氢分解成氧和水的酶,存在于细胞的过氧化物体内。过氧化氢酶是过氧化物酶体的标志酶, 约占过氧化物酶体酶总量的40%。过氧化氢酶存在于所有已知的动物的各个组织中,特别在肝脏中以高浓度存在。过氧化氢酶在食品工业中被用于除去用于制造奶酪的牛奶中的过氧化氢。过氧化氢酶也被用于食品包装,防止食物被氧化。
产品图片
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Placenta tissue lysates
Lane 3: Rat Heart tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-CAT (bs-6874) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated(bs-6874R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (bs-6874R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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