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Rabbit Anti-MST3/STK3  antibody (bs-7599R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7599R
英文名称 MST3/STK3
中文名称 丝氨酸/苏氨酸蛋白激酶3抗体
别    名 Kinase responsive to stress 1; KRS1; Mammalian STE20 like protein kinase 2; Mammalian sterile 20-like 2; Mess1; MST; MST-2; MST2; Mst3; Serine/threonine kinase 3 (STE20 homolog, yeast); Serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine protein kinase 3; Serine/threonine protein kinase Krs1; STE20 like kinase MST2.  
Specific References  (1)     |     bs-7599R has been referenced in 1 publications.
[IF=4.074] Yang J et al. STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. CNS Neurosci Ther. 2020 Aug;26(8):851-861.  IF ;  Human/Mouse.  
研究领域 细胞生物  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Dog,Chicken)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 56kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human STK3: 101-200/491 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation.

Subunit:
Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain).

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain.

SWISS:
Q9Y6E0

Gene ID:
8428

Database links:

Entrez Gene: 8428 Human

Entrez Gene: 223255 Mouse

Entrez Gene: 361092 Rat

Omim: 604984 Human

SwissProt: Q9Y6E0 Human

SwissProt: Q99KH8 Mouse

SwissProt: B0LT89 Rat

Unigene: 508514 Human

Unigene: 390756 Mouse

Unigene: 442081 Mouse

Unigene: 472866 Mouse

Unigene: 105397 Rat



产品图片
Sample:
Muslce (Mouse) Lysate at 40 ug
Fetal brain (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Rat Spleen tissue lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human A431 cell lysates
Lane 7: Human HeLa cell lysates
Primary: Anti-MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 52 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- MST3/STK3 (bs-7599R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MST3 STK3) Polyclonal Antibody, Unconjugated (bs-7599R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-STK3/MST3 Polyclonal Antibody, Unconjugated(bs-7599R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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