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Goat Anti-Rabbit IgG H&L / AF488 antibody (bs-0295G-AF488)

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说明书： 100ul 1ml

100ul/480.00元

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1ml/3000.00元

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产品编号	bs-0295G-AF488
英文名称	Goat Anti-Rabbit IgG H&L / AF488
中文名称	Alexa Fluor 488标记的羊抗兔IgG H&L
别名	Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488); Immunoglobulin G;
	Specific References (57) \| bs-0295G-AF488 has been referenced in 57 publications. [IF=18.808] Ruoping Wang. et al. Sustained Antitumor Immunity Based on Persistent Luminescence Nanoparticles for Cancer Immunotherapy. 2021 Sep 27 IF ; Mouse. PubMed:10.1002/adfm.202106884 [IF=16.836] Ling W et al. Flexible Electronics and Materials for Synchronized Stimulation and Monitoring in Multi‐Encephalic Regions. Adv. Funct. Mater. 2020, 2002644. IHF ; Rabbit. PubMed:10.1002/adfm.202002644 [IF=16.016] Jia-min Yan. et al. Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress. 2021 Nov 06 WB,IF ; Human. PubMed:34747299 [IF=13.903] Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5. IHF ; Rabbit. PubMed:31689086 [IF=13.352] Yu-Ting Zhou. et al. Perfluorooctanoic acid (PFOA) exposure affects early embryonic development and offspring oocyte quality via inducing mitochondrial dysfunction. ENVIRON INT. 2022 Sep;167:107413 IF ; Mouse. PubMed:35863238 [IF=13.281] Yao Li. et al. Antigen Capture and Immune Modulation by Bacterial Outer Membrane Vesicles as In Situ Vaccine for Cancer Immunotherapy Post-Photothermal Therapy. 2022 Feb 12 IF ; Mouse. PubMed:35152555 [IF=12.479] Pan Zhang. et al. The programmed site-specific delivery of LY3200882 and PD-L1 siRNA boosts immunotherapy for triple-negative breast cancer by remodeling tumor microenvironment. BIOMATERIALS. Biomaterials. 2022 Apr;:121518 IF,FC ; Mouse. PubMed:35462305 [IF=11.15] Zhan Yizhi. et al. NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma. BMC MED. 2022 Dec;20(1):1-19 IF ; Human. PubMed:36258210 [IF=10.633] Qianqian Zhang. et al. Engineering a Synergistic Antioxidant Inhibition Nanoplatform to Enhance Oxidative Damage in Tumor Treatment. ACTA BIOMATER. 2023 Jan;: IF ; Human. PubMed:36608895 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119 IF ; Rat. PubMed:36109005 [IF=9.995] Jiang Weiqian. et al. A Peptide RIP Encoded by LncRNA DGCR5 Affecting β-Catenin Subcellular Localization Determines the Differentiation Fate of BMSC in Steroid-Induced ONFH. CELL REP. 2023 Jan 18 IF ; Human. PubMed:10.2139/ssrn.4323728 [IF=9.988] Yu Li. et al. Silibinin supplementation ameliorates the toxic effects of butyl benzyl phthalate on porcine oocytes by eliminating oxidative stress and autophagy. ENVIRON POLLUT. 2023 Jul;329:121734 IF ; Pig. PubMed:37120001 [IF=9.273] Canyu Yang. et al. Optimize the combination regimen of Trastuzumab and Nab-paclitaxel in HER2-positive tumors via modulating Caveolin-1 expression by lovastatin. ASIAN J PHARM SCI. 2022 Jul;: IF ; Human. PubMed:10.1016/j.ajps.2022.06.002 [IF=8.786] Zhaoqi Zhang. et al. Central gene transcriptional regulatory networks shaping monocyte development in bone marrow.. FRONT IMMUNOL. 2022 Oct;13:1011279-1011279 FC ; Mouse. PubMed:36304450 [IF=8.786] Chenchen Xie. et al. USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway. FRONT IMMUNOL. 2023; 14: 1130697 IF ; Mouse. PubMed:37153558 [IF=8.579] Siqi Ying. et al. Low-intensity Pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing Oxidative Stress. Theranostics. 2020; 10(21): 9789–9807 FC ; Mouse, Rat. PubMed:32863960 [IF=8.101] Xiao-Qing Li. et al. Melatonin protects against maternal diabetes-associated meiotic defects by maintaining mitochondrial function. FREE RADICAL BIO MED. 2022 Aug;188:386 IF ; Mouse. PubMed:35792241 [IF=7.666] Yan Liu. et al. S100A8-Mediated NLRP3 Inflammasome-Dependent Pyroptosis in Macrophages Facilitates Liver Fibrosis Progression. CELLS-BASEL. 2022 Jan;11(22):3579 IF ; Human, Mouse. PubMed:36429008 [IF=7.666] Xi Chen. et al. Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling. CELLS-BASEL. 2023 Jan;12(7):1066 ICC ; Human. PubMed:37048139 [IF=6.843] Ning-Ning Chao. et al. Fabrication and characterization of a pro-angiogenic hydrogel derived from the human placenta. Biomater Sci-Uk. 2022 Mar;: IF ; Human Placenta Hydrogels. PubMed:35315457 [IF=6.208] Longcong Dong. et al. Shared Genes of PPARG and NOS2 in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation. INT J MOL SCI. 2023 Jan;24(6):5651 IF ; Mouse. PubMed:36982725 [IF=6.022] Fan Y et al. A function of fascin1 in the colony formation of Mouseembryonic stem cells. Stem Cells. 2020 Sep;38(9):1078-1090. ICF ; Rabbit. PubMed:32379912 [IF=5.241] Yan, Junqiang. et al. Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice. Cell Death Discov. 2022 Jan;8(1):1-8 IF ; Mouse. PubMed:35039479 [IF=5.048] Tingting Hun. et al. A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading. Microsyst Nanoeng. 2020 Sep;6(1):1-13 IF ; Mouse. PubMed:10.1038/s41378-020-00191-5 [IF=4.936] Ma M et al. LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF‐κB/NLRP3 inflammasome pathway.(2018) Cell Prolif. WB ; Rabbit. PubMed:30362186 [IF=4.854] Cheng Wen. et al. Dual response of fibroblasts viability and Porphyromonas gingivalis adhesion on nanostructured zirconia abutment surfaces. J BIOMED MATER RES A. 2022 Jun 08 IF ; Human. PubMed:35676876 [IF=4.831] Lushuang Xie. et al. Inflammatory factors and amyloid β-induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538–22549 IHC ; Rat. PubMed:33196457 [IF=4.627] Xiong X et al. Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem Cell Res Ther. 2019 Dec 18;10(1):396. ICF ; Rabbit. PubMed:31852539 [IF=4.511] W Tang et al. Aligned Biofunctional Electrospun PLGA-LysoGM1 Scaffold for Traumatic Brain Injury Repair. ACS Biomater. Sci. Eng., 19 Feb 2020. IHF ; Rabbit. PubMed:DOI: 10.1021/ acsbiomaterials.9b01636 [IF=4.26] Luo et al. SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells. (2018) Sci.Rep. 8:3912 IF(ICC) ; Rabbit. PubMed:29500367
抗体来源	Goat
克隆类型	Polyclonal
交叉反应	Rabbit,Rat,Mouse,Human
产品应用	IF=1:100-1000, Flow-Cyt=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性状	Liquid
浓度	2.0 mg/ml
免疫原	Native rabbit IgG
亚型	IgG
纯化方法	affinity purified by Protein G, nonspecific adsorbed
缓冲液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
产品图片	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序