| 产品编号 | bs-7645R |
| 英文名称 | DNAJA3 |
| 中文名称 | 肝癌相关抗原57抗体 |
| 别 名 | DnaJ (Hsp40) homolog subfamily A member 3; DnaJ homolog subfamily A member 3 mitochondrial precursor; DnaJ protein Tid 1; DnaJ protein Tid1; HCA57; Hepatocellular carcinoma associated antigen 57; Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME; hTid 1; hTid1; III [Caenorhabditis elegans]; TID1; Tumorous imaginal discs (Drosophila) homolog; Tumorous imaginal discs protein Tid56 homolog; DNJA3_HUMAN. |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TID1/HCA57: 101-200/480 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals. Function: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. Subunit: Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain) (By similarity). Subcellular Location: Mitochondrion matrix. Cytoplasm, cytosol (By similarity). Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein (By similarity). Note=Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK (By similarity). Tissue Specificity: Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes. Post-translational modifications: Tyrosine phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 CR-type zinc finger. Contains 1 J domain. SWISS: Q96EY1 Gene ID: 9093 Database links: Entrez Gene: 9093 Human Entrez Gene: 83945 Mouse Omim: 608382 Human SwissProt: Q96EY1 Human SwissProt: Q99M87 Mouse Unigene: 459779 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
