| 产品编号 | bs-7106R |
| 英文名称 | NUPR1 |
| 中文名称 | 核蛋白p8抗体 |
| 别 名 | Candidate of metastasis 1; COM1; Nuclear protein 1; NUPR1; NUPR1_HUMAN; p8 protein; Protein p8. |
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Specific References (5) | bs-7106R has been referenced in 5 publications.
[IF=24.633] Yanxin Li. et al. Decoding the temporal and regional specification of microglia in the developing human brain. Cell Stem Cell. 2022 Mar;: IF ; Mouse,Human.
[IF=7.126] Narzt et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. (2018) Redox.Biol. 20:467-482 IF ; Human.
[IF=5.402] Li Tan. et al. Age-Related Decline in Expression of Molecular Chaperones Induces Endoplasmic Reticulum Stress and Chondrocyte Apoptosis in Articular Cartilage. Aging Dis. 2020 Oct; 11(5): 1091–1102 IHC ; Monkey.
[IF=5.391] Shiraki M et al. Deficiency of stress-associated gene Nupr1 increases bone volume by attenuating differentiationof osteoclasts and enhancing differentiation of osteoblasts. FASEB J. 2019 May 8:fj201802322RR. IHF ; Mouse.
[IF=2.74] Li Tan. et al. Dietary saturated fatty acid palmitate promotes cartilage lesions and activates the unfolded protein response pathway in mouse knee joints. Plos One. 2021 Feb;16(2):e0247237 IHC ; Mouse.
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| 研究领域 | 细胞生物 染色质和核信号 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 9kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUPR1/p8: 31-82/82 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Could participate in the response to proapoptotic stimuli and promotes cellular growth in a way that helps the tissue counteract diverse injuries. May contribute to the metastatic phenotype.Tissue specificity: Highly expressed in pancreas. Function: Chromatin-binding protein that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Interacts with MSL1 and inhibits its activity on histone H4 'Lys-16' acetylation (H4K16ac). Binds the RELB promoter and activates its transcription, leading to the transactivation of IER3. The NUPR1/RELB/IER3 survival pathway may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. In breast cancer cells, NUPR1 overexpression leads to the activation of PI3K/AKT signaling pathway, CDKN1A/p21 phosphorylation and relocalization from the nucleus to the cytoplasm, leading to resistance to chemotherapeutic agents, such as doxorubicin. Subunit: Monomer. Directly interacts with MSL1 and binds MORF4L1, two components of histone acetyltransferase complex; the interaction with MORF4L1 may be mediated by MSL1. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes. Post-translational modifications: Phosphorylated in vitro by PKA and CK. Phosphorylation promotes DNA-binding activity. Similarity: Belongs to the NUPR family. SWISS: O60356 Gene ID: 26471 Database links: Entrez Gene: 26471 Human SwissProt: O60356 Human Unigene: 418692 Human Unigene: 513463 Human |
| 产品图片 |
Sample:
Lane 1: Human L-O2 cell lysates Lane 2: Human U87MG cell lysates Primary: Anti-NUPR1 (bs-7106R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 9 kDa Observed band size: 11 kDa 
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUPR1) Polyclonal Antibody, Unconjugated (bs-7106R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
