| 产品编号 | bs-7748R |
| 英文名称 | FMN2 Rabbit pAb |
| 中文名称 | 形成素2抗体（成蛋白） |
| 别 名 | FMN 2; FMN-2; Formin2; Formin-2; Formin 2. |
| 研究领域 | 细胞生物 神经生物学 细胞周期蛋白 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 189 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FMN2: 401-500/1722 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FMN2 is a member or the formin homology family, Cappuccino subfamily. Formin homology (FH) domain proteins play a role in cytoskeletal organization and/or establishment of cell polarity. In mice, FMN2 has been shown to be a maternal effect gene that is expressed in oocytes and is required for progression through metaphase of meiosis I. FMN2 is expressed in the developing and mature central nervous system. Subcellular Location: Expressed almost exclusively in the developing and mature central nervous system. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the formin homology family. Cappuccino subfamily. Contains 1 FH1 (formin homology 1) domain. Contains 1 FH2 (formin homology 2) domain. Gene ID: 56776 |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FMN2 Polyclonal Antibody, Unconjugated(bs-7748R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
