| 产品编号 | bs-7849R |
| 英文名称 | Beta tubulin cofactor D Rabbit pAb |
| 中文名称 | 微管蛋白β辅助因子D抗体 |
| 别 名 | beta tubulin cofactor D; beta-tubulin cofactor D; KIAA0988; SSD 1; SSD-1; SSD1; tbcd; TBCD_HUMAN; tfcD; Tubulin folding cofactor D; Tubulin specific chaperone D; Tubulin-folding cofactor D; Tubulin-specific chaperone D. |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞周期蛋白 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Chicken) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 133 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TBCD/Beta tubulin cofactor D: 401-500/1192 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase-activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. Function: Tubulin-folding protein; involved in the first step ofthe tubulin folding pathway. Modulates microtubule dynamics bycapturing GTP-bound beta-tubulin (TUBB). Acts as aGTPase-activating protein (GAP) for ARL2. Its ability to interactwith beta tubulin is regulated via its interaction with ARL2.Induces microtubule disruption in absence of ARL2. Increasesdegradation of beta tubulin, when overexpressed in polarized cells.Promotes epithelial cell detachment, a process antagonized by ARL2.Induces tight adherens and tight junctions disassembly at thelateral cell membrane. Subunit: Found in a complex with at least ARL2, PPP2CB, PPP2R1A,PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB. Interacts withARL2 (By similarity). Supercomplex made of cofactors A to E.Cofactors A and D function by capturing and stabilizing tubulin ina quasi-native conformation. Cofactor E binds to the cofactorD-tubulin complex; interaction with cofactor C then causes therelease of tubulin polypeptides that are committed to the nativestate. Interacts with ARL2; interaction is enhanced with theGDP-bound form of ARL2. Does not interact with ARL3, ARL4A andARL4D. Interacts with beta tubulin. Subcellular Location: Cell junction, tight junction (Bysimilarity). Lateral cell membrane (By similarity). Cytoplasm (Bysimilarity). Cell junction, adherens junction (By similarity).Note=Localized in cell-cell contacts (By similarity). Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the TBCD family. Contains 3 HEAT repeats. SWISS: Q9BTW9.2 Gene ID: 6904 Database links: UniProtKB/Swiss-Prot: Q9BTW9.2 |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin cofactor D (bs-7849R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kD
Observed band size: 133 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
