| 产品编号 | bs-6910R |
| 英文名称 | BMPER Rabbit pAb |
| 中文名称 | BMP内皮细胞调节蛋白抗体 |
| 别 名 | BMP binding endothelial regulator; BMP binding endothelial regulator precursor protein; Bone morphogenetic protein binding endothelial cell precursor derived regulator; CRIM3; Crossveinless 2; CV 2; CV2; hCV2; Protein crossveinless 2; BMPER_HUMAN. |
| 研究领域 | 心血管 细胞生物 免疫学 信号转导 干细胞 生长因子和激素 细胞周期蛋白 转录调节因子 内皮细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 72 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMPER: 501-600/686 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis. [provided by RefSeq, Dec 2011]. BMPER is a BMP-binding protein that is expressed by endothelial cell precursors, has BMP-antagonizing activity, and may play a role in endothelial cell differentiation by modulating local BMP activity. It may also regulate BMP responsiveness in osteoblasts and chondrocytes. Subunit: Interacts with BMP4. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in lung, and brain and also in primary chondrocytes. Similarity: Contains 1 TIL (trypsin inhibitory-like) domain. Contains 5 VWFC domains. Contains 1 VWFD domain. SWISS: Q8N8U9 Gene ID: 168667 Database links: Entrez Gene: 168667 Human Entrez Gene: 73230 Mouse Omim: 608699 Human SwissProt: Q8N8U9 Human SwissProt: Q8CJ69 Mouse Unigene: 660998 Human Unigene: 735847 Human Unigene: 335020 Mouse |
| 产品图片 |
Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-BMPER (bs-6910R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72kD
Observed band size: 75kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPER) Polyclonal Antibody, Unconjugated (bs-6910R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPER) Polyclonal Antibody, Unconjugated (bs-6910R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
