产品编号 | bs-7982R |
英文名称 | HOXC9 Rabbit pAb |
中文名称 | 同源盒蛋白HOXC9抗体 |
别 名 | Homeo box C9; Homeobox C9; Homeobox protein Hox C9; Hox 3B; HOX3; HOX3B. |
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Specific References (1) | bs-7982R has been referenced in 1 publications.
[IF=7] Xu Enbo. et al. Tissue-like cultured fish fillets through a synthetic food pipeline. npj Science of Food. 2023 May;7(1):1-13 ICC ; Fish.
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研究领域 | 细胞生物 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse,Rat (predicted: Sheep,Cow) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 29 kDa |
检测分子量 | |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HOXC9: 151-220/260 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
HOXC9 plays an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subcellular Location: Nucleus. Similarity: Belongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31274 Gene ID: 3225 Database links: Entrez Gene: 3225 Human Entrez Gene: 15427 Mouse Omim: 142971 Human SwissProt: P31274 Human SwissProt: P09633 Mouse Unigene: 658823 Human Unigene: 4765 Mouse |
产品图片 |
Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
Lane 2: Muscle (Rat) Tissue Lysate at 40 ug
Lane 3: Ear (Rat) Tissue Lysate at 40 ug
Primary: Anti-HOXC9 (bs-7982R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 40 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HOXC9 Polyclonal Antibody, Unconjugated(bs-7982R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |