产品编号 | bs-8299R |
英文名称 | TXNRD1 |
中文名称 | 硫氧环蛋白还原酶1抗体 |
别 名 | cytoplasmic; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; Gene associated with retinoid IFN induced mortality 12 protein; GRIM 12; GRIM-12; GRIM12; KDRF; KM 102 derived reductase like factor; KM-102-derived reductase-like factor; MGC9145; Oxidoreductase; Thioredoxin reductase 1; Thioredoxin reductase 1 cytoplasmic; Thioredoxin reductase GRIM 12 antibody Thioredoxin reductase TR1; TR 1; TR; TR1; TRXR 1; TRXR1; TRXR1_HUMAN; TXNR; TXNRD 1; Txnrd1. |
Specific References (4) | bs-8299R has been referenced in 4 publications.
[IF=5.157] Huafeng Wang. et al. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. J BIOL CHEM. 2022 May;:102077 WB ; Mouse.
[IF=5.011] Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436 WB ; Human.
[IF=2.21] Nedret Kilic. et al. Thioredoxin system and miR-21, miR-23a/b and let-7a as potential biomarkers for brain tumor progression: Preliminary Case Data. WORLD NEUROSURG. 2022 Sep;: WB ; Human.
[IF=0] Kaya, Bulent, et al. "Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma." Molecular and Clinical Oncology 5.4 (2016): 343-346. IHC-P ; Human.
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研究领域 | 细胞生物 免疫学 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 71kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TXNRD1: 451-550/649 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. Subunit: Homodimer. Isoform 4 interacts with ESR1 and ESR2. Interacts with HERC5. Subcellular Location: Cytoplasm. Isoform 4: Cytoplasm. Nucleus. Isoform 5: Cytoplasm. Tissue Specificity: Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. Post-translational modifications: The N-terminus of isoform 5 is blocked. ISGylated (Probable). Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. Contains 1 glutaredoxin domain. SWISS: Q16881 Gene ID: 7296 Database links: Entrez Gene: 7296 Human Entrez Gene: 50493 Mouse Omim: 601112 Human SwissProt: Q16881 Human SwissProt: Q9JMH6 Mouse Unigene: 210155 Mouse Unigene: 471159 Mouse Unigene: 67581 Rat |
产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-TXNRD1 (bs-8299R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXNRD1) Polyclonal Antibody, Unconjugated (bs-8299R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TXNRD1 Polyclonal Antibody, Unconjugated(bs-8299R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXNRD1) Polyclonal Antibody, Unconjugated (bs-8299R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TXNRD1) Polyclonal Antibody, Unconjugated (bs-8299R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |