产品编号 | bs-8326R |
英文名称 | Hi95 |
中文名称 | 缺氧诱导基因95抗体 |
别 名 | Hi95; Hypoxia induced gene 95; MGC11758; OTTHUMP00000003641; RP23 63D8.3; SES2; SESN 2; SEST2; Sestrin 2; DKFZp761M02121; OTTMUSP00000010184; DKFZp761M0212; SESN2_HUMAN. |
研究领域 | 心血管 细胞生物 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human (predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 54kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SESN2/Hi95: 101-200/480 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
SESN2 is a member of the sestrin family of PA26-related proteins. The It may function in the regulation of cell growth and survival and also in cellular response to different stress conditions. Function: Involved in the reduction of peroxiredoxins. Subcellular Location: Cytoplasmic and Nuclear Tissue Specificity: Widely expressed. Similarity: Belongs to the sestrin family. SWISS: P58004 Gene ID: 83667 Database links: Entrez Gene: 83667 Human Entrez Gene: 230784 Mouse Omim: 607767 Human SwissProt: P58004 Human SwissProt: P58043 Mouse Unigene: 469543 Human Unigene: 23608 Mouse Unigene: 37672 Rat |
产品图片 |
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (bs-8326R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (bs-8326R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |