| 产品编号 | bs-8414R |
| 英文名称 | HAGHL |
| 中文名称 | 羟基酰谷胱甘肽水解酶样蛋白抗体 |
| 别 名 | HAGHL; HAGHL_HUMAN; Hydroxyacylglutathione hydrolase-like; Hydroxyacylglutathione hydrolase-like protein. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Pig,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAGHL: 1-100/290 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The hydroxyacylglutathione hydrolase-like protein (HAGHL) is a 290 amino acid protein that belongs to the glyoxalase II family. HAGHL binds two zinc ions per subunit and acts as a hydrolase on ester bonds. The gene encoding HAGHL maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The rare disorder Rubinstein-Taybi syndrome is associated with chromosome 16, as is Crohn's disease, a gastrointestinal inflammatory condition that may involve the NOD2 gene. An association with systemic lupus erythematosis and a number of other autoimmune disorders with the pericentromeric region of chromosome 16 has led to the identification of SLC5A11 as a potential autoimmune modifier. Function: Hydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. Subcellular Location: Cytoplasmic and Mitochondrial Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. SWISS: Q6PII5 Gene ID: 84264 Database links: Entrez Gene: 84264 Human SwissProt: Q6PII5 Human Unigene: 124015 Human 克罗恩病(节段性肠炎)Crohn’s disease(segmental enteritis”,曾称:克隆病)的研究。 |
| 产品图片 |
Sample:
Testis(Mouse) Lysate at 40 ug Primary: Anti- HAGHL (bs-8414R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD 
Sample:
MCF-7(Human) Cell Lysate at 40 ug Primary: Anti- HAGHL (bs-8414R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
