[IF=10.787] Limin Shi. et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway. REDOX BIOL. 2023 Jul;63:102727  WB ;  Mouse.  

PubMed:37156218

[IF=3.347] Song Q et al. Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-κB activationToxicol Appl Pharmacol.2021 Jan 1;410:115361.  WB ;  Human.  

PubMed:33285147

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-CD71/Transferrin receptor (bs-0988R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kD
Observed band size: 75 kD

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFRC ) Polyclonal Antibody, Unconjugated (bs-0988R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control(blue):Raji(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- CD71 antibody(bs-0988R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control:K562.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (bs-0988R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.