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Rabbit Anti-Acrosin  antibody (bs-5151R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-5151R
英文名称 Acrosin
中文名称 精子顶体前体蛋白抗体
别    名 ACR; Acrosin precursor; ACRS;Preproacrosin; Proacrosin; ACRO_HUMAN.  
Specific References  (8)     |     bs-5151R has been referenced in 8 publications.
[IF=12.067] Zhang, Guohui. et al. Deficiency of cancer/testis antigen gene CT55 causes male infertility in humans and mice. CELL DEATH DIFFER. 2022 Dec;:1-15  WB, CoIP, IF ;  Mouse, Human.  
[IF=7.81] Deng, Shou‐Long, et al. "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." Journal of Pineal Research (2016).  Sheep.  
[IF=5.168] Deng et al. Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORα signaling in anin vitrogoat spermatogonial stem cell differentiation culture system. (2017) Oncotarget. 8:110592-110605  IF(ICC) ;  Goat.  
[IF=4.87] Li Y et al. Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. Mol Cell Proteomics. 2020 Jun;19(6):1035-1046.  WB ;  Chicken.  
[IF=3.412] Liu Y et al. Fluoride interferes with sperm fertilizing ability via downregulated SPAM1, ACR and PRSS21 expression in rat epididymis. J Agric Food Chem. 2019 May 8;67(18):5240-5249.  WB ;  Rat.  
[IF=3.396] Yu K et al. In-vitro differentiation of early pig spermatogenic cells to haploid germ cells. Mol Hum Reprod. 2019 Jul 22. pii: gaz043.  
[IF=3.23] Liang, Xin, et al. "The Noncircadian Function of the Circadian Clock Gene in the Regulation of Male Fertility." Journal of biological rhythms 28.3 (2013): 208-217.  
[IF=2.775] Ru Yu. et al. ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice. 2021 Dec 13  WB,IHC ;  Mouse,Human.  
研究领域 细胞生物  免疫学  泛素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep)
产品应用 WB=1:500-2000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 33/44kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Acrosin heavy chain: 201-300/421 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Function:
Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Subunit:
Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms heterodimer with SERPINA5.

Similarity:
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.

SWISS:
P10323

Gene ID:
49

Database links:

Entrez Gene: 49 Human

Omim: 102480 Human

SwissProt: P10323 Human



产品图片
Sample:
Epididymis (Mouse) Lysate at 40 ug
Primary: Anti-Acrosin (bs-5151R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/44kD
Observed band size: 33 kD
Blank control(black line):HepG2.
Primary Antibody (green line): Rabbit Anti-Acrosin antibody (bs-5151R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Acrosin antibody (bs-5151R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:HUVEC.
Primary Antibody (green line): Rabbit Anti-Acrosin antibody (bs-5151R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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