| 产品编号 | bs-6455R |
| 英文名称 | CHRNA3 Rabbit pAb |
| 中文名称 | 烟碱型乙酰胆碱受体α3抗体 |
| 别 名 | Nicotinic Acetylcholine Receptor alpha 3; ACHA3_HUMAN; Cholinergic receptor neuronal nicotinic alpha polypeptide 3; Cholinergic receptor nicotinic alpha 3; Cholinergic receptor nicotinic alpha polypeptide 3; CHRNA 3; CHRNA3; CHRNA-3; NACHRA 3; NACHRA3; Neuronal acetylcholine receptor protein alpha 3 chain precursor; Neuronal acetylcholine receptor subunit alpha 3; Neuronal acetylcholine receptor subunit alpha-3; Neuronal nicotinic acetylcholine receptor alpha 3 subunit. |
| 研究领域 | 细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHRNA3/AChRα3: 101-200/505 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChR?, also known as LNCR2, PAOD2, NACHRA3 or CHRNA3, is a 505 amino acid multi-pass membrane protein that belongs to the ligand-gated ion channel receptor family and may play a role in neurotransmission. Function: After binding acetylcholine, the AChR responds by anextensive change in conformation that affects all subunits andleads to opening of an ion-conducting channel across the plasmamembrane. Subunit: Neuronal AChR is composed of two different types ofsubunits: alpha and beta. Alpha-3 subunit can be combined to beta-2or beta-4 to give rise to functional receptors. Interacts withRIC3; which is required for proper folding and assembly. Subcellular Location: Cell junction, synapse, postsynaptic cellmembrane; Multi-pass membrane protein. Cell membrane; Multi-passmembrane protein. Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9)family. Acetylcholine receptor (TC 1.A.9.1) subfamily.Alpha-3/CHRNA3 sub-subfamily. SWISS: P32297 Gene ID: 1136 Database links: Entrez Gene: 1136 Human Entrez Gene: 110834 Mouse Omim: 118503 Human SwissProt: P32297 Human SwissProt: Q8R4G9 Mouse Unigene: 89605 Human Unigene: 63569 Mouse Unigene: 10996 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
