| 产品编号 | bs-9432R |
| 英文名称 | GERP |
| 中文名称 | 环指蛋白27抗体 |
| 别 名 | RNF27; Lioblastoma expressed ring finger protein; Ring finger protein 27; RNF27; RP11-47A8.4; TRIM8; Tripartite motif containing 8; Tripartite motif protein TRIM8; TRIM8_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GERP/TRIM8/RNF27: 61-160/551 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM8 (tripartite motif containing 8), also known as GERP (glioblastoma-expressed RING finger protein) or RNF27 (RING finger protein 27), is a 551 amino acid protein that is thought to function as an E3 ubiquitin-protein ligase that promotes SOCS-1 proteasomal degradation. As a widely expressed homodimer, TRIM8 localizes to nuclear bodies and contains two B box-type zinc fingers and one RING-type zinc finger. TRIM8 is expressed in lung, heart, brain and skeletal muscle, with low levels detected in intestine, placenta, leukocytes and liver. The gene encoding TRIM8 maps to human chromosome 10q24.32. Function: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Subunit: Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box. Tissue Specificity: Widely expressed. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger. SWISS: Q9BZR9 Gene ID: 81603 Database links: Entrez Gene: 81603 Human Entrez Gene: 93679 Mouse Omim: 606125 Human SwissProt: Q9BZR9 Human SwissProt: Q99PJ2 Mouse Unigene: 336810 Human Unigene: 392177 Mouse Unigene: 481672 Mouse 葛博：bs-9432R 细胞定位：细胞质 细胞核 2017.11.08日张凤英修改 |
| 产品图片 |
Sample:
Placenta (Mouse) Lysate at 40 ug Primary: Anti- GERP (bs-9432R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD 
Sample:
HepG2 Cell (Human) Lysate at 30 ug Hela Cell(Human)Lysate at 30 ug Primary: Anti- GERP (bs-9432R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
