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Rabbit Anti-FEZ1  antibody (bs-9721R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-9721R
英文名称 FEZ1
中文名称 脑胚胎锌指样蛋白1抗体
别    名 Fasciculation and elongation protein zeta 1; Fasciculation and elongation protein zeta-1; Fez 1; Fez; Fez-like zinc finger protein; FEZ1; FEZ1_HUMAN; Fezl; Forebrain embryonic zinc-finger like protein; Zpf312; Zygin 1; Zygin I; Zygin-1; Zygin1; ZyginI.  
研究领域 细胞生物  免疫学  神经生物学  细胞周期蛋白  细胞分化  锌指蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse (predicted: Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 45kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FEZ1: 301-392/392 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Frequent loss of heterozygosity (LOH) at human chromosome 8p22-p21 is associated with various tumors including prostate and breast cancer. The 8p22-p21 region contains the FEZ1 gene, which is altered in tumors of the esophagus, prostate and breast. The FEZ1 protein (also known as leucine zipper putative tumor suppressor or LZTS1) contains a DNA-binding leucine zipper motif. FEZ1 is expressed in normal breast and prostate, but alterations in FEZ1 expression result in abnormal cell growth. The absence of FEZ1 expression is characteristic of breast and prostate cancer cell lines as well as primary breast and pro-state tumors. This absence of FEZ1 may be due to several factors, including mutations in the FEZ1 gene or hypermethylation of the CpG island flanking the FEZ1 promoter region. FEZ1 acts as a negative regulator of cell growth. During cell-cycle progression, FEZ1 localizes to microtubule components and is hyperphosphorylated by cAMP-dependent kinase.

Function:
May be involved in axonal outgrowth as component of thenetwork of molecules that regulate cellular morphology and axonguidance machinery. Able to restore partial locomotion and axonalfasciculation to C.elegans unc-76 mutants in germlinetransformation experiments. May participate in the transport ofmitochondria and other cargos along microtubules.

Subunit:
Homodimer; difulfide-linked. May form heterodimers withFEZ2. Interacts with the NH2-terminal variable region (V1) of PKCzeta and weakly with that of PKC epsilon (By similarity). Interactswith UBE4B. Interacts with SAP30L. Interacts with SCOC and ULK1;SCOC interferes with ULK1-binding to FEZ1. Directly interacts withSCOC and UVRAG. Stabilizes the interaction between SCOC and UVRAGduring amino acid starvation.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Cellmembrane (By similarity). Note=Co-localizes with both, alpha- andgamma-tubulin. Translocated from the plasma membrane to thecytoplasm by activation of the PKC zeta (By similarity).

Tissue Specificity:
Mainly expressed in brain.

Post-translational modifications:
Phosphorylated by protein kinase C zeta; which enhancesinteraction with UBE4B and polyubiquitination.
Polyubiquitinated in a UBE4B-dependent manner; which does notlead to proteasomal degradation and may be important for neurogenicactivity. Polyubiquitin linkage seems to be mainly through Lys-26.

Similarity:
Belongs to the zygin family.

SWISS:
Q99689

Gene ID:
9638

Database links:

Entrez Gene: 9638 Human

Entrez Gene: 235180 Mouse

Entrez Gene: 81730 Rat

Omim: 604825 Human

SwissProt: Q99689 Human

SwissProt: Q8K0X8 Mouse

SwissProt: P97577 Rat

Unigene: 224008 Human

Unigene: 5264 Mouse

Unigene: 5468 Rat



产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- FEZ1 (bs-9721R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FEZ1) Polyclonal Antibody, Unconjugated (bs-9721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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