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Rabbit Anti-3-Nitrotyrosine  antibody (bs-8551R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-8551R
英文名称 3-Nitrotyrosine
中文名称 硝基酪氨酸/硝基化酪氨酸抗体
别    名 NO tyrosine; nTyr; Nitrotyrosine; nitro-Tyrosine; nitro Tyrosine.  酪氨酸硝基化;
Specific References  (14)     |     bs-8551R has been referenced in 14 publications.
[IF=18.027] Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX  IHC ;  Mouse.  
[IF=15.881] Yuan Chen. et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis. Acs Nano. 2022;XXXX(XXX):XXX-XXX  IHC ;  Mouse.  
[IF=14.588] Yan Xuet al. Blockade of Platelets Using Tumor-Specific NO-Releasing Nanoparticles Prevents Tumor Metastasis and Reverses Tumor Immunosuppression. ACS Nano . 2020 Aug 25;14(8):9780-9795.  IF ;  mouse.  
[IF=5.78] Li, Dawei, et al. "Hepatic Hypoxia-Inducible Factors Inhibit PPARα Expression To Exacerbate Acetaminophen induced Oxidative Stress And Hepatotoxicity." Free Radical Biology and Medicine (2017).  IHC-P ;  Mouse.  
[IF=4.868] Feng Y et al. Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway. Oxid Med Cell Longev. 2019 Nov 6;2019:7067619.  IHC-P ;  Mouse.  
[IF=4.52] Ahmed S et al. Cocoa Flavonoids Reduce Inflammation and Oxidative Stress in a Myocardial Ischemia-Reperfusion Experimental Model. Antioxidants (Basel). 2020 Feb 18;9(2). pii: E167.  IHC-P ;  Rat.  
[IF=4.38] Chen, Yu-Lin, and Wai-Ming Kan. "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2015).  Human.  
[IF=3.75] Chuang Yang. et al. Gastrodin protects endothelial cells against high glucose-induced injury through up-regulation of PPARβ and alleviation of nitrative stress. MICROVASC RES. 2023 Jul;148:104531  WB ;  Human.  
[IF=3.514] Chuang Yang. et al. PPARβ down-regulation is involved in high glucose-induced endothelial injury via acceleration of nitrative stress. Microvasc Res. 2022 Jan;139:104272  WB ;   Rat.  
[IF=3.146] Wang, Lin-Feng, et al. "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." American Journal of Translational Research 7.10 (2015): 1724-1735.  IHC-P ;  Mouse.  
[IF=3.04] Tawfik KM et al. Neuroprotective mechanisms of sildenafil and selenium in PTZ-kindling model: Implications in epilepsy.Eur J Pharmacol. 2018 Aug 15;833:131-144.  IHC-P ;  Mouse.  
[IF=2.998] Canonica J et al. Effect of acute and chronic aldosterone exposure on the retinal pigment epithelium-choroid complex in rodents. Exp Eye Res. 2019 Aug 5;187:107747.  IHC ;  Mouse.  
[IF=2.43] Zhang, Jing-Yao, et al. "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." World Journal of Gastroenterology 21.14 (2015): 4195-4209.  IHC-P ;  Mouse.  
[IF=2.05] Li et al. Targeting intestinal epithelial cell-programmed necrosis alleviates tissue injury after intestinal ischemia/reperfusion in rats. (2018) J.Surg.Res. 225:108-117  IHC ;  Mouse.  
产品类型 小分子抗体 特殊修饰抗体 
研究领域 肿瘤  细胞生物  信号转导  Alzheimer's  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Nitrotyrosine,Rat,Human (predicted: Mouse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Nitrotyrosine 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.

Subcellular Location:
Cytoplasm.

SWISS:
N/A

CAS:
621-44-3

产品图片
Sample: 3-Nitrotyrosine-BSA conjugate Protein
Primary:Anti-3-Nitrotyrosine (bs-8551R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nitro tyrosine Polyclonal Antibody, Unconjugated(bs-8551R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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