产品编号 | bs-0957R |
英文名称 | PAG608 |
中文名称 | 野生型P53诱导基因1抗体 |
别 名 | WIG-1; p53 activated gene 608; PAG608; WIG1; FLJ12296; MGC10613; OTTHUMP00000211906; OTTHUMP00000211907; p53-activated gene 608 protein; PAG 608; WIG 1; Zinc finger matrin-type protein 3; Zinc finger protein WIG-1; ZMAT3_HUMAN; p53 inducible zinc finger protein; p53 target zinc finger protein; PAG 608; WIG 1; WIG 1/PAG608 protein; FLJ31683; WIG1; Zinc finger matrin-type protein 3; WIG1; WIG1/PAG608 protein; Zinc finger matrin type 3; Zinc finger protein WIG 1; Zinc finger protein WIG1; ZMAT 3; ZMAT3. |
研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat (predicted: Mouse,Chicken,Dog,Cow,Horse,GuineaPig) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 32kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human WIG-1: 51-150/289 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a protein containing three zinc finger domains and a nuclear localization signal. The mRNA and the protein of this gene are upregulated by wildtype p53 and overexpression of this gene inhibits tumor cell growth, suggesting that this gene may have a role in the p53-dependent growth regulatory pathway. Alternative splicing of this gene results in two transcript variants encoding two isoforms differing in only one amino acid. Function: Acts as a bona fide target gene of p53/TP53. May play a role in the TP53-dependent growth regulatory pathway. May contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Subunit: Interacts with dsRNA. Subcellular Location: Nucleus. Nucleus, nucleolus. Tissue Specificity: Highly expressed in adult brain, and moderately in adult kidney and testis. Not detected in fetal brain, heart, pancreas, adrenal gland, liver or small intestine. Similarity: Contains 3 matrin-type zinc fingers. SWISS: Q9HA38 Gene ID: 64393 Database links: Entrez Gene: 64393 Human Entrez Gene: 22401 Mouse Omim: 606452 Human SwissProt: Q9HA38 Human SwissProt: O54836 Mouse Unigene: 371609 Human Unigene: 35705 Mouse Unigene: 2925 Rat Tissue Specificity : Highly expressed in adult brain, and moderately in adult kidney and testis. Not detected in fetal brain, heart, pancreas, adrenal gland, liver or small intestine. |
产品图片 |
Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug Lane 2: Huvec (Human) Cell Lysate at 30 ug Lane 3: U87MG (Human) Cell Lysate at 30 ug Lane 4: U2os (Human) Cell Lysate at 30 ug Primary: Anti- PAG608 (bs-0957R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAG608) Polyclonal Antibody, Unconjugated (bs-0957R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAG608) Polyclonal Antibody, Unconjugated (bs-0957R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |