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Rabbit Anti-p47/NSFL1C antibody (bs-9902R)
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说明书: 100ul  200ul
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-9902R
英文名称 p47/NSFL1C
中文名称 血小板47蛋白抗体
别    名 DJ776F14.1; MGC3347; NSF1C_HUMAN; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; NSFL1C; OTTHUMP00000029972; OTTHUMP00000029974; p47; P47 protein; P97 cofactor p47; SHP1 homolog; UBX domain protein 2C; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C.  
研究领域 心血管  细胞生物  信号转导  生长因子和激素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 40kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Pleckstrin/p47:21-120/350 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Platelet P47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

Function:
Major protein kinase C substrate of platelets.

Subcellular Location:
Nucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks.

Tissue Specificity:
Major protein kinase C substrate of platelets.

Post-translational modifications:
Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.

Similarity:
Contains 1 DEP domain.
Contains 2 PH domains.

SWISS:
P08567

Gene ID:
5341

Database links:

Entrez Gene: 5341 Human

Entrez Gene: 56193 Mouse

Omim: 173570 Human

SwissProt: Q6Q308 Dog

SwissProt: P08567 Human

SwissProt: Q9JHK5 Mouse

Unigene: 468840 Human

Unigene: 98232 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- p47’NSFL1C (bs-9902R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p47 NSFL1C) Polyclonal Antibody, Unconjugated (bs-9902R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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