| 产品编号 | bs-8554R |
| 英文名称 | Hemoglobin Beta |
| 中文名称 | 血红蛋白β抗体 |
| 别 名 | Beta 1 globin; beta globin; beta globin chain; BETA GLOBIN TYPE; BETA THALASSEMIAS; CD113t C; CD113t-C; ERYTHREMIA; HBB; Hemoglobin beta 1 chain; hemoglobin beta chain; hemoglobin beta chain complex; Hemoglobin beta chain, major form; HEMOGLOBIN BETA LOCUS; METHEMOGLOBINEMIA |
| 研究领域 | 心血管 细胞生物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15.5kDa |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hemoglobin beta: 51-147/147 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]. Function: Involved in oxygen transport from the lung to the various peripheral tissues. Tissue Specificity: Red blood cells. Post-translational modifications: The initiator Met is not cleaved in variant Thionville and is acetylated. DISEASE: Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency. Similarity: Belongs to the globin family. SWISS: P68871 Gene ID: 3043 Database links: Entrez Gene: 3043 Human Omim: 141900 Human SwissProt: P68871 Human Unigene: 523443 Human |
| 产品图片 |
Sample:
K562(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Hemoglobin Beta (bs-8554R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15.5 kD Observed band size: 25.5 kD 
Sample:
MOLT-4(Human) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti-Hemoglobin Beta (bs-8554R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15.5 kD Observed band size: 25.5 kD 
Sample:
K562(Human) Cell Lysate at 30 ug Primary: Anti-Hemoglobin Beta (bs-8554R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15.5 kD Observed band size: 15.5 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
